PMID- 17079742
OWN - NLM
STAT- MEDLINE
DCOM- 20070412
LR  - 20161124
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 28
IP  - 2
DP  - 2007 Mar-Apr
TI  - Differential expression of neurotrophins in penises of streptozotocin-induced 
      diabetic rats.
PG  - 306-12
AB  - To explore the mechanism of diabetic erectile dysfunction, we studied the 
      distribution of neurotrophins in the penises of diabetic rats, including nerve 
      growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin-3 
      (NT-3), and neurotrophin-4 (NT-4). Male Sprague-Dawley rats were injected with 65 
      mg/kg streptozotocin to induce diabetes mellitus (DM). The control rats were 
      raised as age-matched control. Eight weeks later, the intercavernous pressure 
      (ICP) of the rats was measured after electrostimulation and before sacrifice. 
      Each peeled penis was divided into 2 parts, one for immunohistochemistry and the 
      other for Western blot analysis. The ICP of the DM group rats was significantly 
      decreased as compared to the vehicle control rats. There were significantly more 
      NGF-positive neurons in the penises of the diabetic rats than in those of the 
      control rats, while the opposite results were observed for BDNF-positive neurons. 
      In the Western blot analysis, the proteins of NGF, NT-3, and NT-4 were all 
      increased, while that of BDNF was decreased in diabetic rats. This is the first 
      study revealing the expression of NT-4 protein in cavernous tissue. The abnormal 
      level of these 4 neurotrophins in cavernous tissue may be one of the factors of 
      the pathogenesis of diabetic ED. The increase of neurotrophins may reflect the 
      degree of cavernous tissue denervation and may represent a compensatory 
      mechanism. The lesion of the retrograde axonal transport of the nerves caused by 
      hyperglycemia may be related to this phenomenon.
FAU - Chen, Yun
AU  - Chen Y
AD  - Department of Urology, Affiliated Drum Tower Hospital, Nanjing University, School 
      of Medicine, Nanjing 210008, China.
FAU - Yang, Rong
AU  - Yang R
FAU - Yao, Leshen
AU  - Yao L
FAU - Sun, Zeyu
AU  - Sun Z
FAU - Wang, Run
AU  - Wang R
FAU - Dai, Yutian
AU  - Dai Y
LA  - eng
PT  - Journal Article
DEP - 20061101
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 5W494URQ81 (Streptozocin)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/biosynthesis
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Down-Regulation
MH  - Erectile Dysfunction/chemically induced
MH  - Gene Expression/drug effects
MH  - Male
MH  - Nerve Growth Factor/biosynthesis
MH  - Nerve Growth Factors/*biosynthesis
MH  - Neurotrophin 3/biosynthesis
MH  - Penis/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Streptozocin/pharmacology
MH  - Up-Regulation
EDAT- 2006/11/03 09:00
MHDA- 2007/04/14 09:00
CRDT- 2006/11/03 09:00
PHST- 2006/11/03 09:00 [pubmed]
PHST- 2007/04/14 09:00 [medline]
PHST- 2006/11/03 09:00 [entrez]
AID - jandrol.106.000794 [pii]
AID - 10.2164/jandrol.106.000794 [doi]
PST - ppublish
SO  - J Androl. 2007 Mar-Apr;28(2):306-12. doi: 10.2164/jandrol.106.000794. Epub 2006 
      Nov 1.