PMID- 17080504
OWN - NLM
STAT- MEDLINE
DCOM- 20070226
LR  - 20191210
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 20
IP  - 12
DP  - 2006 Dec
TI  - A simple and sensitive HPLC-fluorescence method for quantification of MDMA and 
      MDA in blood with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a 
      label.
PG  - 1380-5
AB  - A sensitive high-performance liquid chromatographic method with fluorescence 
      detection to determine 3,4-methylenedioxymethamphethamine (MDMA) and 
      3,4-methylenedioxyamphethamine (MDA) in human and rat whole blood or plasma 
      samples was developed by using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride 
      (DIB-Cl) as a label. MDMA and MDA in a small amount of blood sample (ca 100 
      microL) were extracted by liquid-liquid extraction with ethyl acetate, and were 
      derivatized with DIB-Cl under mild conditions (10 min at room temperature). A 
      good separation of DIB-derivatives could be achieved within 45 min using a 
      commercially available ODS column with an isocratic eluent of 10 mM citric 
      acid-20 mM Na(2)HPO(4) aqueous buffer (pH 4.0)-CH(3)CN-CH(3)OH (50:45:5, v/v/v 
      %). The calibration curves prepared with 1-methyl-3-phenylpropylamine (MPPA) as 
      an internal standard showed good linearity (r = 0.999) with 0.36-0.83 ng/mL 
      detection limit at a signal-to-noise ratio of 3. MDMA and MDA in rat whole blood 
      could be monitored for 6 h after a single administration of MDMA (2.2 mg/kg, 
      i.p.). The pharmacokinetic parameters for MDMA and MDA obtained by triplicate 
      measurements were 426 +/- 23 and 39 +/- 6 ng/mL (C(max)), 20 +/- 5 and 100 +/- 10 
      min (T(max)), respectively.
CI  - Copyright (c) 2006 John Wiley & Sons, Ltd.
FAU - Tomita, Mamoru
AU  - Tomita M
AD  - Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate 
      School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 
      852-8521, Japan.
FAU - Nakashima, Mihoko N
AU  - Nakashima MN
FAU - Wada, Mitsuhiro
AU  - Wada M
FAU - Nakashima, Kenichiro
AU  - Nakashima K
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride)
RN  - 0 (Benzoates)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Imidazoles)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*blood
MH  - Animals
MH  - Benzoates/chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Fluorescence
MH  - Fluorescent Dyes/chemistry
MH  - Humans
MH  - Imidazoles/chemistry
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*blood
MH  - Rats
EDAT- 2006/11/03 09:00
MHDA- 2007/02/27 09:00
CRDT- 2006/11/03 09:00
PHST- 2006/11/03 09:00 [pubmed]
PHST- 2007/02/27 09:00 [medline]
PHST- 2006/11/03 09:00 [entrez]
AID - 10.1002/bmc.716 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2006 Dec;20(12):1380-5. doi: 10.1002/bmc.716.