PMID- 17080932
OWN - NLM
STAT- MEDLINE
DCOM- 20070112
LR  - 20220317
IS  - 0140-7791 (Print)
IS  - 0140-7791 (Linking)
VI  - 29
IP  - 6
DP  - 2006 Jun
TI  - Senescence-specific regulation of catalases in Arabidopsis thaliana (L.) Heynh.
PG  - 1049-60
AB  - Oxygen free radicals are thought to play an essential role in senescence, 
      especially those derived from peroxisomes. Therefore, the activities of different 
      isoforms of the peroxisomal hydrogen peroxide (H2O2)-scavenging enzyme catalase 
      (CAT) were analysed during senescence of Arabidopsis. CAT2 activity decreased 
      with bolting time parallel with cytosolic ascorbate peroxidase 1 (APX1) activity 
      before loss of chlorophyll could be measured. At the same time point, the H2O2 
      content increased. Subsequently, the stress-inducible CAT3 isoform was activated 
      and APX1 activity was recovered, accompanied by a decline of the H2O2 content. In 
      very late stages, low activities of the seed-specific CAT1 became detectable in 
      leaves, but H2O2 increased again. Further analyses of CAT expression by promoter: 
      beta-glucuronidase (GUS) fusions in transgenic plants revealed a 
      vasculature-specific CAT3 expression, whereas CAT2 expression turned out to be 
      specific for photosynthetic active tissues. CAT2 expression is down-regulated 
      during leaf senescence, while CAT3 expression is induced with age and corresponds 
      to an accumulation of H2O2 in the vascular bundles. CAT2 down-regulation on the 
      transcriptional level appears as the initial step in creating the H2O2 peak 
      during bolting time, while the decrease in APX1 activity might only be a 
      secondary and amplifying effect.
FAU - Zimmermann, Petra
AU  - Zimmermann P
AD  - ZMBP (Centre of Molecular Biology of Plants), University of Tubingen, Auf der 
      Morgenstelle 28, 72076 Tubingen, Germany.
FAU - Heinlein, Christina
AU  - Heinlein C
FAU - Orendi, Gabriele
AU  - Orendi G
FAU - Zentgraf, Ulrike
AU  - Zentgraf U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plant Cell Environ
JT  - Plant, cell & environment
JID - 9309004
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Herbicides)
RN  - 0 (Isoenzymes)
RN  - 0 (Recombinant Fusion Proteins)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (CAT3 protein, Arabidopsis)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.11 (Ascorbate Peroxidases)
RN  - EC 1.11.1.6 (CAT2 protein, Arabidopsis)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 3.2.1.31 (Glucuronidase)
RN  - PLG39H7695 (Paraquat)
SB  - IM
MH  - Arabidopsis/cytology/*enzymology/genetics
MH  - Arabidopsis Proteins/antagonists & inhibitors/genetics/metabolism/physiology
MH  - Ascorbate Peroxidases
MH  - Catalase/genetics/*metabolism/physiology
MH  - Cellular Senescence/*genetics
MH  - Gene Expression Regulation, Plant
MH  - Glucuronidase/analysis
MH  - Herbicides/pharmacology
MH  - Hydrogen Peroxide/analysis/metabolism/pharmacology
MH  - Isoenzymes/genetics/metabolism/physiology
MH  - Oxidative Stress/genetics
MH  - Paraquat/pharmacology
MH  - Peroxidases/antagonists & inhibitors/metabolism
MH  - Peroxisomes/enzymology
MH  - Plant Leaves/enzymology
MH  - Plants, Genetically Modified/metabolism
MH  - Recombinant Fusion Proteins/analysis
EDAT- 2006/11/04 09:00
MHDA- 2007/01/16 09:00
CRDT- 2006/11/04 09:00
PHST- 2006/11/04 09:00 [pubmed]
PHST- 2007/01/16 09:00 [medline]
PHST- 2006/11/04 09:00 [entrez]
AID - 10.1111/j.1365-3040.2005.01459.x [doi]
PST - ppublish
SO  - Plant Cell Environ. 2006 Jun;29(6):1049-60. doi: 
      10.1111/j.1365-3040.2005.01459.x.