PMID- 1708341
OWN - NLM
STAT- MEDLINE
DCOM- 19910530
LR  - 20181130
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 21
IP  - 4
DP  - 1991 Apr
TI  - Differential activation of human basophils by anti-IgE and 
      formyl-methionyl-leucyl-phenylalanine. Indications for protein kinase C-dependent 
      and -independent activation pathways.
PG  - 881-5
AB  - Upon activation, basophilic granulocytes release inflammatory mediators such as 
      histamine. We studied histamine release of human basophils (64.1 +/- 9.6% pure) 
      after cross-linking of membrane-bound IgE via anti-IgE, or after binding of the 
      chemoattractant formyl-methionyl-leucyl-phenylalanine (fMLP). A variability in 
      the extent of histamine release upon stimulation by either anti-IgE or fMLP was 
      found between donors. Kinetic studies revealed that the histamine release induced 
      by anti-IgE (t1/2 greater than 240 s) was more than 20-fold slower than the 
      almost instantaneous release upon stimulation with fMLP (t1/2 less than 10 s). 
      Differences in the cell activation pathways triggered by these stimuli were 
      further analyzed with staurosporine, an inhibitor of protein kinase C (PKC) and 
      with wortmannin, an inhibitor of a PKC-independent pathway. Inhibition of PKC 
      resulted in a partial inhibition of the anti-IgE-induced release, whereas the 
      fMLP-induced release was slightly potentiated. The anti-IgE-induced release was 
      completely inhibited in the presence of wortmannin. This inhibitor too, had no 
      effect on the fMLP-induced release. We conclude that major differences exist in 
      the signal-response coupling between the anti-IgE and fMLP-induced histamine 
      release in human basophils. The so-called releasability of human basophils may be 
      due to the availability of different cell activation pathways.
FAU - Knol, E F
AU  - Knol EF
AD  - Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, 
      Amsterdam.
FAU - Koenderman, L
AU  - Koenderman L
FAU - Mul, F P
AU  - Mul FP
FAU - Verhoeven, A J
AU  - Verhoeven AJ
FAU - Roos, D
AU  - Roos D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Alkaloids)
RN  - 0 (Androstadienes)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - H88EPA0A3N (Staurosporine)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Alkaloids/pharmacology
MH  - Androstadienes/pharmacology
MH  - Antibodies, Anti-Idiotypic/*immunology
MH  - Basophils/drug effects/*metabolism
MH  - Cells, Cultured
MH  - *Histamine Release/drug effects
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Protein Kinase C/*physiology
MH  - Staurosporine
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Wortmannin
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 10.1002/eji.1830210404 [doi]
PST - ppublish
SO  - Eur J Immunol. 1991 Apr;21(4):881-5. doi: 10.1002/eji.1830210404.