PMID- 17083466
OWN - NLM
STAT- MEDLINE
DCOM- 20070730
LR  - 20131121
IS  - 0950-382X (Print)
IS  - 0950-382X (Linking)
VI  - 62
IP  - 6
DP  - 2006 Dec
TI  - Identification of an essential gene responsible for D-Asp incorporation in the 
      Lactococcus lactis peptidoglycan crossbridge.
PG  - 1713-24
AB  - Bacteria such as Lactococcus lactis have D-aspartate (D-Asp) or its amidated 
      derivative D-asparagine (D-Asn), in their peptidoglycan (PG) interpeptide 
      crossbridge. We performed a subtractive genome analysis to identify L. lactis 
      gene yxbA, orthologues of which being present only in bacteria containing D-amino 
      acids in their PG crossbridge, but absent from those that instead insert L-amino 
      acids or glycine. Inactivation of yxbA required a complementing Streptococcus 
      pneumoniae murMN genes, which express enzymes that incorporate L-Ser-L-Ala or 
      L-Ala-L-Ala in the PG crossbridge. Our results show that (i) yxbA encodes D-Asp 
      ligase responsible for incorporation of D-Asp in the PG crossbridge, and we 
      therefore renamed it as aslA, (ii) it is an essential gene, which makes its 
      product a potential target for specific antimicrobials, (iii) the absence of 
      D-Asp may be complemented by L-Ser-L-Ala or L-Ala-L-Ala in the L. lactis PG, 
      indicating that the PG synthesis machinery is not selective for the side-chain 
      residues, and (iv) lactococcal strains having L-amino acids in their PG 
      crossbridge display defects in cell wall integrity, but are able to efficiently 
      anchor cell wall proteins, indicating relative flexibility of lactococcal 
      transpeptidation reactions with respect to changes in PG sidechain composition.
FAU - Veiga, Patrick
AU  - Veiga P
AD  - Unite Bacteries Lactiques et Pathogenes Opportunistes, INRA, 78352 Jouy-en-Josas 
      Cedex France.
FAU - Piquet, Sandra
AU  - Piquet S
FAU - Maisons, Aurelie
AU  - Maisons A
FAU - Furlan, Sylviane
AU  - Furlan S
FAU - Courtin, Pascal
AU  - Courtin P
FAU - Chapot-Chartier, Marie-Pierre
AU  - Chapot-Chartier MP
FAU - Kulakauskas, Saulius
AU  - Kulakauskas S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
RN  - 0 (Bacterial Proteins)
RN  - 0 (Peptidoglycan)
RN  - 30KYC7MIAI (Aspartic Acid)
SB  - IM
MH  - Aspartic Acid/chemistry/*metabolism
MH  - Bacterial Proteins/chemistry/genetics/metabolism
MH  - Blotting, Western
MH  - Cell Division/genetics/physiology
MH  - Cell Wall/chemistry/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Gene Order
MH  - *Genes, Bacterial
MH  - *Genes, Essential
MH  - Genome, Bacterial
MH  - In Situ Hybridization, Fluorescence
MH  - Lactococcus lactis/*genetics/metabolism/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Mutation
MH  - Operon
MH  - Peptidoglycan/chemistry/*metabolism
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2006/11/07 09:00
MHDA- 2007/07/31 09:00
CRDT- 2006/11/07 09:00
PHST- 2006/11/07 09:00 [pubmed]
PHST- 2007/07/31 09:00 [medline]
PHST- 2006/11/07 09:00 [entrez]
AID - MMI5474 [pii]
AID - 10.1111/j.1365-2958.2006.05474.x [doi]
PST - ppublish
SO  - Mol Microbiol. 2006 Dec;62(6):1713-24. doi: 10.1111/j.1365-2958.2006.05474.x.