PMID- 17083537
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20171116
IS  - 1395-3907 (Print)
IS  - 1395-3907 (Linking)
VI  - 84
IP  - 6
DP  - 2006 Dec
TI  - Donor-derived myofibroblasts in the ocular surface after allogeneic 
      haematopoietic stem cell transplantation.
PG  - 774-80
AB  - PURPOSE: To identify and characterize cells of donor origin in the ocular surface 
      of female recipients who have undergone allogeneic haematopoietic stem cell 
      transplantation (allo-SCT) from a male donor. METHODS: Cytological impressions 
      from the eyes of nine allografted patients (17 eyes) were analysed. Donor cells 
      were identified using sex-chromosome-specific fluorescence in situ hybridization 
      (FISH). Cells were characterized by immunohistochemistry (IHC) using the CK3 and 
      CK19 epithelial markers, the panleucocytic marker CD45 and the myofibroblast 
      marker alpha-SMA. RESULTS: No epithelial cells of donor origin were observed in 
      the corneal or conjunctival samples. Cells of donor origin were found in the 
      corneal samples, although these were often too degraded to allow characterization 
      by IHC. In the conjunctiva, a median of 86% of the total number of cells were of 
      recipient origin, including a subgroup (2%) of giant cells exhibiting polyploidy 
      (range 4-18 n), found in the limbal region. Donor cells were detected in the 
      conjunctiva of all nine patients at a median ratio of 9%, of which two-thirds 
      were CD45+/alpha-SMA+. CONCLUSIONS: We observed superficially located 
      myofibroblasts of donor origin in all allografted patients, but not in samples 
      from healthy controls. Whether myofibroblasts are implicated in ocular 
      graft-versus-host disease requires further studies.
FAU - Hallberg, David
AU  - Hallberg D
AD  - Department of Ophthalmology, Sahlgrenska University Hospital/Molndal, Gothenburg, 
      Sweden.
FAU - Wernstedt, Pernilla
AU  - Wernstedt P
FAU - Hanson, Charles
AU  - Hanson C
FAU - Wettergren, Yvonne
AU  - Wettergren Y
FAU - Stenberg, Kristina
AU  - Stenberg K
FAU - Brune, Mats
AU  - Brune M
FAU - Stenevi, Ulf
AU  - Stenevi U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Acta Ophthalmol Scand
JT  - Acta ophthalmologica Scandinavica
JID - 9507578
RN  - 0 (Actins)
RN  - 0 (Biomarkers)
RN  - 0 (KRT3 protein, human)
RN  - 0 (Keratin-19)
RN  - 0 (Keratin-3)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Actins/metabolism
MH  - Adult
MH  - Biomarkers/metabolism
MH  - Chromosomes, Human, X/metabolism
MH  - Chromosomes, Human, Y/metabolism
MH  - Conjunctiva/*cytology/metabolism
MH  - Cornea/*cytology/metabolism
MH  - Female
MH  - Fibroblasts/*cytology/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Keratin-19/metabolism
MH  - Keratin-3/metabolism
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy
MH  - Leukemia, Myeloid, Acute/therapy
MH  - Leukocyte Common Antigens/metabolism
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Tissue Donors
MH  - Transplantation, Homologous
EDAT- 2006/11/07 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/11/07 09:00
PHST- 2006/11/07 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/11/07 09:00 [entrez]
AID - AOS748 [pii]
AID - 10.1111/j.1600-0420.2006.00748.x [doi]
PST - ppublish
SO  - Acta Ophthalmol Scand. 2006 Dec;84(6):774-80. doi: 
      10.1111/j.1600-0420.2006.00748.x.