PMID- 17083712
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20191210
IS  - 0266-8254 (Print)
IS  - 0266-8254 (Linking)
VI  - 43
IP  - 6
DP  - 2006 Dec
TI  - Separation of yeast cells from aqueous solutions using magnetically stabilized 
      fluidized beds.
PG  - 652-8
AB  - AIMS: To separate Saccharomyces cerevisiae cells from aqueous solutions using 
      magnetically stabilized fluidized beds (MSFB) that utilize a horizontal magnetic 
      field, and to study the effect of some parameters, such as bed porosity and 
      height, liquid flow rate and inlet concentration on cell removal efficiency and 
      breakthrough curves. METHODS AND RESULTS: The separation process was conducted in 
      an MSFB under the effect of horizontal magnetic field. The magnetic particles 
      used consist of a ferromagnetic core of magnetite (Fe3O4) covered by a stable 
      layer of activated carbon to adsorb the yeast cells from the suspension. The 
      yeast cell concentration in the effluent was determined periodically by measuring 
      the absorbance at 610 nm. The effect of the magnetic field intensity on the bed 
      porosity and consequently the exit-normalized cell concentration from the bed was 
      studied. It was found that bed porosity increased by 75%, and the normalized cell 
      concentration in the bed effluent decreased by 30%, when the magnetic field 
      intensity was increased from 0 to 110 mT. In addition, increasing the magnetic 
      field intensity and bed height delayed the breakthrough point, and allowed 
      efficient cell removal. These results demonstrate an improved method to separate 
      cells of low concentration from cell suspension. CONCLUSIONS: This study allows 
      the continuous separation of yeast cells from aqueous solutions in an MSFB. The 
      removal efficiency is affected by different parameters including the bed height, 
      flow rate and initial concentration. The removal efficiency reaches 82%, and 
      could be improved by varying the operational parameters. SIGNIFICANCE AND IMPACT 
      OF THE STUDY: The results obtained in this investigation show that the MSFB using 
      horizontal fields represents a potential tool for the continuous separation of 
      cell suspension from aqueous solution. This study will contribute to a better 
      understanding of the hydrodynamic parameters on the separation efficiencies of 
      the cell.
FAU - Al-Qodah, Z
AU  - Al-Qodah Z
AD  - Department of Chemical Engineering, Al-Balqa Applied University, Amman, Jordan. 
      z_Alqodah@hotmail.com
FAU - Al-Shannag, M
AU  - Al-Shannag M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Solutions)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Cell Separation/*instrumentation/*methods
MH  - *Magnetics
MH  - Saccharomyces cerevisiae/*isolation & purification
MH  - Solutions
MH  - Water
EDAT- 2006/11/07 09:00
MHDA- 2007/02/28 09:00
CRDT- 2006/11/07 09:00
PHST- 2006/11/07 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2006/11/07 09:00 [entrez]
AID - LAM2004 [pii]
AID - 10.1111/j.1472-765X.2006.02004.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2006 Dec;43(6):652-8. doi: 10.1111/j.1472-765X.2006.02004.x.