PMID- 17083784
OWN - NLM
STAT- MEDLINE
DCOM- 20100803
LR  - 20160607
IS  - 0578-1310 (Print)
IS  - 0578-1310 (Linking)
VI  - 44
IP  - 8
DP  - 2006 Aug
TI  - [Effects of glutamine on matrix metalloproteinase-3 and tissue inhibitor of 
      metalloproteinase-3 expressions in myocardium of rats with sepsis].
PG  - 587-91
AB  - OBJECTIVE: The underlying mechanisms for cardiac dysfunction in sepsis include 
      the inhibitory effect of endotoxin and inflammatory factors on myocardium and the 
      decrease in cardiac myocardial cells in number. However, whether there is 
      ventricular remodeling resulted from the abnormalities of extracellular collagen 
      metabolism and whether glutamine (Gln) can protect myocardium from LPS-induced 
      damage as in reperfusion are unknown. The aim of the present study was to examine 
      the effects of Gln on the expressions of matrix metalloproteinase-3 (MMP-3), 
      tissue inhibitor of metalloproteinase-3 (TIMP-3) and their mRNA in myocardium of 
      rats with sepsis. METHODS: Classical rat model of sepsis was established by 
      intraperitoneal injection of lipopolysaccharide (LPS) (4 mg/kg, from Escherichia 
      coli O(55): B(5), Sigma). from 121 Wistar rats aged 18 days were divided into 
      three groups randomly, 0 h control group (normal saline: 1 ml/kg, n = 11), LPS 
      group (LPS: 4 mg/kg, n = 55) and Gln group (LPS: 4 mg/kg and immediately 13.64% 
      glutamine 1 ml/kg, Fresenus, n = 55). Furthermore, LPS and Gln groups were 
      examined at 2 h, 4 h, 6 h, 24 h and 72 h time points (n = 11). On each time 
      point, rats of LPS and Gln groups as well as control group were anesthetized with 
      1% chloral hydrate injected intraperitoneally at a dosage of 1 ml/kg. Then, rats 
      were sacrificed, and the hearts were isolated. Eight of them were frozen at minus 
      80 degrees C to measure the expression of TIMP-3 mRNA by using RT-PCR. The 
      expressions of MMP-3 and TIMP-3 were observed with immunohistochemistry and the 
      expression of MMP-3 mRNA was observed by using in situ hybridization. RESULTS: 
      (1) Compared to 0 h, the mRNA expressions of MMP-3 and TIMP-3 in LPS group 
      significantly increased (P < 0.01) with the peak at 6 - 24 h. While, in Gln 
      group, they were significantly higher than those in controls but significantly 
      lower than those in LPS group with the peak at 24 h (P < 0.01). Even at 72 h, 
      they were still higher than those at 0 h (P < 0.05 and P < 0.01). (2) Compared to 
      0 h, the expressions of MMP-3 and TIMP-3 in LPS group were significantly lower at 
      any other time point with the lowest at 6 h (P < 0.01). In Gln group, these 
      expressions were also significantly lower than those in controls, but 
      significantly higher than those in LPS group with the lowest being postponed to 
      24 h (P < 0.01). (3) The ultra structure changed obviously. Z line was unclear 
      and the ridge of mitochondrion disappeared. While, in Gln group, the myocardial 
      injury was slight compared to that in LPS group. CONCLUSIONS: MMP-3 mRNA 
      expression was increased and TIMP-3 mRNA expression was depressed in LPS-induced 
      sepsis. Myocardial extracellular matrix was damaged in sepsis. Glutamine might 
      decrease the effects of LPS on MMP-3 and TIMP-3 expressions and postpone the time 
      of myocardial matrix injury.
FAU - Wang, Hong
AU  - Wang H
AD  - Department of Pediatrics, 2nd Affiliated Hospital, China Medical University, 
      Shenyang 110004, China.
FAU - Yu, Xian-yi
AU  - Yu XY
FAU - Sun, Mei
AU  - Sun M
FAU - Pan, Jing-kun
AU  - Pan JK
FAU - Gao, Hong
AU  - Gao H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Ke Za Zhi
JT  - Zhonghua er ke za zhi = Chinese journal of pediatrics
JID - 0417427
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 0RH81L854J (Glutamine)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Disease Models, Animal
MH  - Female
MH  - Glutamine/administration & dosage/*pharmacology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Lipopolysaccharides/toxicity
MH  - Male
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Myocardium/cytology/metabolism
MH  - Myocytes, Cardiac/*drug effects/*metabolism/ultrastructure
MH  - RNA, Messenger/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sepsis/complications/*drug therapy/genetics/metabolism
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinase-3/*metabolism
EDAT- 2006/11/07 09:00
MHDA- 2010/08/04 06:00
CRDT- 2006/11/07 09:00
PHST- 2006/11/07 09:00 [pubmed]
PHST- 2010/08/04 06:00 [medline]
PHST- 2006/11/07 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Ke Za Zhi. 2006 Aug;44(8):587-91.