PMID- 17084491
OWN - NLM
STAT- MEDLINE
DCOM- 20070314
LR  - 20070108
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 25
IP  - 7
DP  - 2007 Jan 26
TI  - Immune enhancing effects of recombinant bovine IL-18 on foot-and-mouth disease 
      vaccination in mice model.
PG  - 1257-64
AB  - Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed 
      animals and can cause a considerable socio-economic loss for affected countries. 
      Interleukin-18 (IL-18) is a pleiotropic cytokine and plays important role in both 
      the development of a functional immune system as well as the response of the 
      organism to infection. In the present study, bovine IL-18 (BoIL-18), 
      Foot-and-mouth disease virus VP1 and VP1/BoIL-18 fusion genes were cloned and 
      expressed in pichia pastoris (P. pastoris) and subsequently immune effects were 
      evaluated to study the immune enhancing effects of recombinant BoIL-18 (rBoIL-18) 
      on FMD vaccination. The results showed that the genes encoding for BoIL-18, VP1 
      and VP1/BoIL-18 are successfully expressed in P. pastoris and the expressed 
      recombinant VP1 (rVP1) proteins could induce both humoral and marginal 
      cell-mediated immune responses in mice, while the co-inoculation with rBoIL-18 
      could markedly enhance both of immune responses, and the inoculation of the 
      fusion product rVP1/BoIL-18 showed even more dramatic immune responses, 
      suggesting rBoIL-18 has a potential to enhance the efficacy of vaccination 
      against FMDV infection.
FAU - Shi, Xi-Ju
AU  - Shi XJ
AD  - College of Veterinary Medicine, China Agricultural University, Beijing 100094, PR 
      China.
FAU - Wang, Bin
AU  - Wang B
FAU - Wang, Ming
AU  - Wang M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061017
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Animals
MH  - Blotting, Western
MH  - Cattle
MH  - Cell Proliferation
MH  - Cloning, Molecular
MH  - Cytokines/biosynthesis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Foot-and-Mouth Disease/*immunology/*prevention & control
MH  - Foot-and-Mouth Disease Virus/*immunology
MH  - Hypersensitivity, Delayed/immunology
MH  - Immunization
MH  - Immunoglobulin G/biosynthesis
MH  - Indicators and Reagents
MH  - Interleukin-18/*pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Pichia/genetics
MH  - Recombinant Proteins/pharmacology
MH  - T-Lymphocytes/drug effects/immunology
MH  - Th1 Cells/metabolism
MH  - Th2 Cells/metabolism
MH  - Vaccines, Synthetic/immunology
MH  - Viral Vaccines/*immunology
EDAT- 2006/11/07 09:00
MHDA- 2007/03/16 09:00
CRDT- 2006/11/07 09:00
PHST- 2006/08/29 00:00 [received]
PHST- 2006/09/29 00:00 [revised]
PHST- 2006/10/04 00:00 [accepted]
PHST- 2006/11/07 09:00 [pubmed]
PHST- 2007/03/16 09:00 [medline]
PHST- 2006/11/07 09:00 [entrez]
AID - S0264-410X(06)01108-X [pii]
AID - 10.1016/j.vaccine.2006.10.017 [doi]
PST - ppublish
SO  - Vaccine. 2007 Jan 26;25(7):1257-64. doi: 10.1016/j.vaccine.2006.10.017. Epub 2006 
      Oct 17.