PMID- 17085711
OWN - NLM
STAT- MEDLINE
DCOM- 20070525
LR  - 20240315
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 73
IP  - 1
DP  - 2007 Jan
TI  - Communities of archaea and bacteria in a subsurface radioactive thermal spring in 
      the Austrian Central Alps, and evidence of ammonia-oxidizing Crenarchaeota.
PG  - 259-70
AB  - Scanning electron microscopy revealed great morphological diversity in biofilms 
      from several largely unexplored subterranean thermal Alpine springs, which 
      contain radium 226 and radon 222. A culture-independent molecular analysis of 
      microbial communities on rocks and in the water of one spring, the 
      "Franz-Josef-Quelle" in Bad Gastein, Austria, was performed. Four hundred fifteen 
      clones were analyzed. One hundred thirty-two sequences were affiliated with 14 
      bacterial operational taxonomic units (OTUs) and 283 with four archaeal OTUs. 
      Rarefaction analysis indicated a high diversity of bacterial sequences, while 
      archaeal sequences were less diverse. The majority of the cloned archaeal 16S 
      rRNA gene sequences belonged to the soil-freshwater-subsurface (1.1b) 
      crenarchaeotic group; other representatives belonged to the 
      freshwater-wastewater-soil (1.3b) group, except one clone, which was related to a 
      group of uncultivated Euryarchaeota. These findings support recent reports that 
      Crenarchaeota are not restricted to high-temperature environments. Most of the 
      bacterial sequences were related to the Proteobacteria (alpha, beta, gamma, and 
      delta), Bacteroidetes, and Planctomycetes. One OTU was allied with Nitrospina sp. 
      (delta-Proteobacteria) and three others grouped with Nitrospira. Statistical 
      analyses suggested high diversity based on 16S rRNA gene analyses; the 
      rarefaction plot of archaeal clones showed a plateau. Since Crenarchaeota have 
      been implicated recently in the nitrogen cycle, the spring environment was probed 
      for the presence of the ammonia monooxygenase subunit A (amoA) gene. Sequences 
      were obtained which were related to crenarchaeotic amoA genes from marine and 
      soil habitats. The data suggested that nitrification processes are occurring in 
      the subterranean environment and that ammonia may possibly be an energy source 
      for the resident communities.
FAU - Weidler, Gerhard W
AU  - Weidler GW
AD  - Division of Molecular Biology, Department of Microbiology, University of 
      Salzburg, Billrothstrasse 11, A-5020 Salzburg, Austria.
FAU - Dornmayr-Pfaffenhuemer, Marion
AU  - Dornmayr-Pfaffenhuemer M
FAU - Gerbl, Friedrich W
AU  - Gerbl FW
FAU - Heinen, Wolfgang
AU  - Heinen W
FAU - Stan-Lotter, Helga
AU  - Stan-Lotter H
LA  - eng
SI  - GENBANK/AM039528
SI  - GENBANK/AM039529
SI  - GENBANK/AM039530
SI  - GENBANK/AM039531
SI  - GENBANK/AM039532
SI  - GENBANK/AM039533
SI  - GENBANK/AM039534
SI  - GENBANK/AM039535
SI  - GENBANK/AM039536
SI  - GENBANK/AM039537
SI  - GENBANK/AM039538
SI  - GENBANK/AM039539
SI  - GENBANK/AM039540
SI  - GENBANK/AM039541
SI  - GENBANK/AM039542
SI  - GENBANK/AM039543
SI  - GENBANK/AM039544
SI  - GENBANK/AM039545
SI  - GENBANK/AM039546
SI  - GENBANK/AM039547
SI  - GENBANK/AM039548
SI  - GENBANK/AM039549
SI  - GENBANK/AM039550
SI  - GENBANK/AM233905
SI  - GENBANK/AM260487
SI  - GENBANK/AM260488
SI  - GENBANK/AM260489
GR  - P 19250/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061103
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
RN  - Q74S4N8N1G (Radon)
RN  - W90AYD6R3Q (Radium)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Archaea/*classification/genetics/growth & development
MH  - Austria
MH  - Bacteria/*classification/genetics/growth & development
MH  - Biofilms/growth & development
MH  - Crenarchaeota/classification/isolation & purification
MH  - DNA, Archaeal/analysis
MH  - DNA, Bacterial/analysis
MH  - Ecosystem
MH  - Hot Springs/chemistry/*microbiology
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Oxidoreductases/genetics
MH  - Proteobacteria/classification/genetics/growth & development
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Radium
MH  - *Radon
MH  - Sequence Analysis, DNA
PMC - PMC1797131
EDAT- 2006/11/07 09:00
MHDA- 2007/05/26 09:00
PMCR- 2007/05/01
CRDT- 2006/11/07 09:00
PHST- 2006/11/07 09:00 [pubmed]
PHST- 2007/05/26 09:00 [medline]
PHST- 2006/11/07 09:00 [entrez]
PHST- 2007/05/01 00:00 [pmc-release]
AID - AEM.01570-06 [pii]
AID - 1570-06 [pii]
AID - 10.1128/AEM.01570-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2007 Jan;73(1):259-70. doi: 10.1128/AEM.01570-06. Epub 
      2006 Nov 3.