PMID- 17086173
OWN - NLM
STAT- MEDLINE
DCOM- 20061228
LR  - 20220311
IS  - 1087-0156 (Print)
IS  - 1087-0156 (Linking)
VI  - 24
IP  - 11
DP  - 2006 Nov
TI  - Reversal of mouse hepatic failure using an implanted liver-assist device 
      containing ES cell-derived hepatocytes.
PG  - 1412-9
AB  - Severe acute liver failure, even when transient, must be treated by 
      transplantation and lifelong immune suppression. Treatment could be improved by 
      bioartificial liver (BAL) support, but this approach is hindered by a shortage of 
      human hepatocytes. To generate an alternative source of cells for BAL support, we 
      differentiated mouse embryonic stem (ES) cells into hepatocytes by coculture with 
      a combination of human liver nonparenchymal cell lines and fibroblast growth 
      factor-2, human activin-A and hepatocyte growth factor. Functional hepatocytes 
      were isolated using albumin promoter-based cell sorting. ES cell-derived 
      hepatocytes expressed liver-specific genes, secreted albumin and metabolized 
      ammonia, lidocaine and diazepam. Treatment of 90% hepatectomized mice with a 
      subcutaneously implanted BAL seeded with ES cell-derived hepatocytes or primary 
      hepatocytes improved liver function and prolonged survival, whereas treatment 
      with a BAL seeded with control cells did not. After functioning in the BAL, ES 
      cell-derived hepatocytes developed characteristics nearly identical to those of 
      primary hepatocytes.
FAU - Soto-Gutierrez, Alejandro
AU  - Soto-Gutierrez A
AD  - Department of Surgery, Okayama University Graduate School of Medicine and 
      Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
FAU - Kobayashi, Naoya
AU  - Kobayashi N
FAU - Rivas-Carrillo, Jorge David
AU  - Rivas-Carrillo JD
FAU - Navarro-Alvarez, Nalu
AU  - Navarro-Alvarez N
FAU - Zhao, Debiao
AU  - Zhao D
FAU - Okitsu, Teru
AU  - Okitsu T
FAU - Noguchi, Hirofumi
AU  - Noguchi H
FAU - Basma, Hesham
AU  - Basma H
FAU - Tabata, Yashuhiko
AU  - Tabata Y
FAU - Chen, Yong
AU  - Chen Y
FAU - Tanaka, Kimiaki
AU  - Tanaka K
FAU - Narushima, Michiki
AU  - Narushima M
FAU - Miki, Atsushi
AU  - Miki A
FAU - Ueda, Tadayoshi
AU  - Ueda T
FAU - Jun, Hee-Sook
AU  - Jun HS
FAU - Yoon, Ji-Won
AU  - Yoon JW
FAU - Lebkowski, Jane
AU  - Lebkowski J
FAU - Tanaka, Noriaki
AU  - Tanaka N
FAU - Fox, Ira J
AU  - Fox IJ
LA  - eng
GR  - DK48794/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061105
PL  - United States
TA  - Nat Biotechnol
JT  - Nature biotechnology
JID - 9604648
RN  - 0 (activin A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 104625-48-1 (Activins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
EIN - Nat Biotechnol. 2007 Feb;25(2):254. Zhao, Debaio [corrected to Zhao, Debiao]
CIN - Hepatology. 2007 Mar;45(3):829-30. PMID: 17326214
MH  - Activins/physiology
MH  - Animals
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/*physiology
MH  - Cell Transplantation/methods/*physiology
MH  - Embryonic Stem Cells/*physiology/*transplantation
MH  - Fibroblast Growth Factor 2/physiology
MH  - Hepatocyte Growth Factor/physiology
MH  - Hepatocytes/cytology/*physiology
MH  - Humans
MH  - Liver Failure, Acute/*therapy
MH  - *Liver, Artificial
MH  - Mice
MH  - Models, Animal
EDAT- 2006/11/07 09:00
MHDA- 2006/12/29 09:00
CRDT- 2006/11/07 09:00
PHST- 2006/08/18 00:00 [received]
PHST- 2006/10/06 00:00 [accepted]
PHST- 2006/11/07 09:00 [pubmed]
PHST- 2006/12/29 09:00 [medline]
PHST- 2006/11/07 09:00 [entrez]
AID - nbt1257 [pii]
AID - 10.1038/nbt1257 [doi]
PST - ppublish
SO  - Nat Biotechnol. 2006 Nov;24(11):1412-9. doi: 10.1038/nbt1257. Epub 2006 Nov 5.