PMID- 17088377
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 72
IP  - 11
DP  - 2006 Nov
TI  - Genetic analysis of bacteriocin 43 of vancomycin-resistant Enterococcus faecium.
PG  - 6955-64
AB  - A total of 636 vancomycin-resistant Enterococcus faecium (VRE) isolates obtained 
      between 1994 and 1999 from the Medical School Hospital of the University of 
      Michigan were tested for bacteriocin production. Of the 277 (44%) 
      bacteriocinogenic strains, 21 were active against E. faecalis, E. faecium, E. 
      hirae, E. durans, and Listeria monocytogenes. Of those 21 strains, a 
      representative bacteriocin of strain VRE82, designated bacteriocin 43, was found 
      to be encoded on mobilizable plasmid pDT1 (6.2 kbp). Nine open reading frames 
      (ORFs), ORF1 to ORF9, were presented on pDT1 and were oriented in the same 
      direction. The bacteriocin 43 locus (bac43) consists of the bacteriocin gene bacA 
      (ORF1) and the immunity gene bacB (ORF2). The deduced bacA product is 74 amino 
      acids in length with a putative signal peptide of 30 amino acids at the N 
      terminus. The bacB gene encodes a deduced 95-amino-acid protein without a signal 
      sequence. The predicted mature BacA protein (44 amino acids) showed sequence 
      homology with the membrane-active class IIa bacteriocins of lactic acid bacteria 
      and showed 86% homology with bacteriocin 31 from E. faecalis YI717 and 98% 
      homology with bacteriocin RC714. Southern analysis with a bac43 probe of each 
      plasmid DNA from the 21 strains showed hybridization to a specific fragment 
      corresponding to the 6.2-kbp EcoRI fragment, suggesting that the strains harbored 
      the pDT1-like plasmid (6.2 kb) which encoded the bacteriocin 43-type bacteriocin. 
      The bac43 determinant was not identified among non-VRE clinical isolates.
FAU - Todokoro, Daisuke
AU  - Todokoro D
AD  - Department of Bacteriology and Bacterial Infection Control, Gunma University 
      Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
FAU - Tomita, Haruyoshi
AU  - Tomita H
FAU - Inoue, Takako
AU  - Inoue T
FAU - Ike, Yasuyoshi
AU  - Ike Y
LA  - eng
SI  - GENBANK/AB178871
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060821
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Bacteriocins)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (bacA protein, Enterococcus)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacteriocins/*biosynthesis/chemistry/*genetics/pharmacology
MH  - Base Sequence
MH  - DNA Transposable Elements
MH  - Enterococcus/classification/drug effects
MH  - Enterococcus faecium/drug effects/genetics/*metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Open Reading Frames/genetics
MH  - Plasmids
MH  - Sequence Analysis, DNA
MH  - *Vancomycin Resistance
PMC - PMC1636183
EDAT- 2006/11/08 09:00
MHDA- 2007/01/24 09:00
PMCR- 2007/03/01
CRDT- 2006/11/08 09:00
PHST- 2006/11/08 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/11/08 09:00 [entrez]
PHST- 2007/03/01 00:00 [pmc-release]
AID - AEM.00934-06 [pii]
AID - 0934-06 [pii]
AID - 10.1128/AEM.00934-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2006 Nov;72(11):6955-64. doi: 10.1128/AEM.00934-06. Epub 
      2006 Aug 21.