PMID- 17089341
OWN - NLM
STAT- MEDLINE
DCOM- 20070112
LR  - 20161124
IS  - 0899-0042 (Print)
IS  - 0899-0042 (Linking)
VI  - 19
IP  - 1
DP  - 2007 Jan
TI  - Stereoselective determination of benalaxyl in plasma by chiral high-performance 
      liquid chromatography with diode array detector and application to 
      pharmacokinetic study in rabbits.
PG  - 51-5
AB  - A chiral high-performance liquid chromatography method with diode array detector 
      was developed and validated for stereoselective determination of benalaxyl (BX) 
      in rabbit plasma. Good separation was achieved at 20 degrees C using cellulose 
      tris-(3,5-dimethylphenylcarbamate) as chiral stationary phase, a mixture of 
      n-hexane and 2-propanol (97:3) as mobile phase at a flow rate of 1.0 ml/min. The 
      assay method was linear over a range of concentrations (0.25-25 microg/ml) in 
      plasma and the mean recovery was greater than 90% for both enantiomers. The 
      limits of quantification and detection for both enantiomers in plasma were 0.25 
      and 0.1 microg/ml, respectively. Intra- and interday relative standard deviations 
      (RSDs) did not exceed 10% for three-tested concentrations. The method was 
      successfully applied to pharmacokinetic studies of BX enantiomers in rabbits. The 
      result suggested that the pharmacokinetics of BX enantiomers was stereoselective 
      in rabbits.
CI  - (c) 2006 Wiley-Liss, Inc.
FAU - Qiu, Jing
AU  - Qiu J
AD  - Department of Applied Chemistry, China Agricultural University, Beijing 100094, 
      China.
FAU - Wang, Qiuxia
AU  - Wang Q
FAU - Zhu, Wentao
AU  - Zhu W
FAU - Jia, Guifang
AU  - Jia G
FAU - Wang, Xinquan
AU  - Wang X
FAU - Zhou, Zhiqiang
AU  - Zhou Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Chirality
JT  - Chirality
JID - 8914261
RN  - 18TH6NY90J (benalaxyl)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
MH  - Alanine/*analogs & derivatives/blood/pharmacokinetics
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Male
MH  - Rabbits
MH  - Stereoisomerism
EDAT- 2006/11/08 09:00
MHDA- 2007/01/16 09:00
CRDT- 2006/11/08 09:00
PHST- 2006/11/08 09:00 [pubmed]
PHST- 2007/01/16 09:00 [medline]
PHST- 2006/11/08 09:00 [entrez]
AID - 10.1002/chir.20341 [doi]
PST - ppublish
SO  - Chirality. 2007 Jan;19(1):51-5. doi: 10.1002/chir.20341.