PMID- 17091509
OWN - NLM
STAT- MEDLINE
DCOM- 20070130
LR  - 20151119
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 108
IP  - 6
DP  - 2006 Dec 25
TI  - An online quiz uncovers limitations of morphology in equivocal lung cytology.
PG  - 480-7
AB  - BACKGROUND: Equivocal atypia in respiratory cytology can be a diagnostic 
      challenge. In such cases fluorescence in situ hybridization (FISH) may be used 
      for the analysis of chromosomal aberrations and often allows a reliable 
      distinction of benign and malignant cells. METHODS: An online picture gallery of 
      30 respiratory cytologic preparations comprising 23 specimens with equivocal 
      cytology as well as 5 positive and 2 negative controls was prepared 
      (www.unibas.ch/patho/lungenzyto/loesung). The final diagnoses were confirmed by 
      clinical follow-up or biopsy or both. Each of the illustrated cell groups was 
      analyzed by multitarget FISH after PAP image capturing and automatic 
      relocalization. RESULTS: The online questionnaire was completed by 137 
      cytomorphologists from all continents. The control cases were assessed accurately 
      to a significantly higher percentage than the equivocal cases. In equivocal cases 
      participants more often made false-positive than false-negative diagnoses. In 2 
      patients with benign conditions (tuberculosis and pulmonary capillaritis) the 
      rate of false-positive answers was remarkably high (31.4% and 62.8% 
      respectively). The result of the 20 best-performing participants for the 5 cases 
      with the highest percentage of inaccurate answers was not better than if they had 
      chosen their answer by chance. CONCLUSIONS: These data illustrate that single 
      cells or cell clusters of a subgroup of equivocal lung cytology are a diagnostic 
      challenge even for highly experienced morphologists. Internet-based tests are 
      able to reveal limitations of cytomorphology.
FAU - Glatz, Katharina
AU  - Glatz K
AD  - Department for Pathology, Institute for Pathology, University Hospital, Basel, 
      Switzerland. katharina.glatz@unibas.ch
FAU - Savic, Spasenija
AU  - Savic S
FAU - Glatz, Dieter
AU  - Glatz D
FAU - Francz, Gilbert
AU  - Francz G
FAU - Barascud, Audrey
AU  - Barascud A
FAU - Grilli, Bruno
AU  - Grilli B
FAU - Herzog, Michelle
AU  - Herzog M
FAU - Dalquen, Peter
AU  - Dalquen P
FAU - Feichter, Georg
AU  - Feichter G
FAU - Spieler, Peter
AU  - Spieler P
FAU - Tamm, Michael
AU  - Tamm M
FAU - Bubendorf, Lukas
AU  - Bubendorf L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Non-Small-Cell Lung/genetics/*pathology
MH  - Carcinoma, Small Cell/genetics/*pathology
MH  - *Chromosome Aberrations
MH  - Cytodiagnosis
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung/*pathology
MH  - Lung Neoplasms/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Online Systems/*organization & administration
MH  - Predictive Value of Tests
MH  - Surveys and Questionnaires
EDAT- 2006/11/09 09:00
MHDA- 2007/01/31 09:00
CRDT- 2006/11/09 09:00
PHST- 2006/11/09 09:00 [pubmed]
PHST- 2007/01/31 09:00 [medline]
PHST- 2006/11/09 09:00 [entrez]
AID - 10.1002/cncr.22286 [doi]
PST - ppublish
SO  - Cancer. 2006 Dec 25;108(6):480-7. doi: 10.1002/cncr.22286.