PMID- 17092279
OWN - NLM
STAT- MEDLINE
DCOM- 20070322
LR  - 20191210
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 99
IP  - 2
DP  - 2007 Feb
TI  - Renal cell carcinoma sub-typing by histopathology and fluorescence in situ 
      hybridization on a needle-biopsy specimen.
PG  - 290-5
AB  - OBJECTIVE: To determine the subtype of renal cell carcinoma (RCC) on needle-core 
      biopsies of renal masses using histopathology and fluorescence in situ 
      hybridization (FISH), and to evaluate the use of interphase FISH to augment the 
      accuracy of needle-core biopsies. PATIENTS AND METHODS: Histology correlates with 
      prognosis in RCC but, historically, biopsies are inaccurate for histological 
      subtype. As histological subtypes of RCC have distinct cytogenetic abnormalities 
      (loss of 3p in clear cell, trisomy 7 or 17 in papillary and widespread 
      chromosomal losses in chromophobe), we hypothesized that FISH would improve the 
      accuracy of biopsies. Forty patients with renal masses underwent nephrectomy, 
      yielding 42 tumours. Needle-core biopsies were taken of the mass immediately 
      after surgery. Interphase FISH was performed on one core for chromosomes 3, 7, 
      10, 13, 17, and 21 and the locus 3p25-26. Histopathology was performed on a 
      second core. Results were compared in a 'blinded' fashion with final pathology. 
      RESULTS: In all, 36 of 42 masses were RCC or oncocytoma. Histopathology of the 
      biopsy correctly identified the tumour subtype in 27 (75%), while four (11%) were 
      incorrectly classified and five (14%) were inadequate for diagnosis. With the 
      addition of FISH, 31 (86%) were correctly subtyped, while two (6%) were incorrect 
      and three (8%) were inadequate. In cases with adequate tissue, histology alone 
      was 87% accurate, while the combined method was 94% accurate. CONCLUSION: 
      Needle-core biopsy of renal tumours provides adequate material for evaluation of 
      histological subtype. Adding FISH to histopathology might improve the accuracy of 
      kidney tumour biopsies, providing important prognostic information that can guide 
      management decisions.
FAU - Barocas, Daniel A
AU  - Barocas DA
AD  - Department of Urology, New York Presbyterian Hospital, Weill Cornell Medical 
      Center, New York, NY, USA.
FAU - Mathew, Susan
AU  - Mathew S
FAU - DelPizzo, Joseph J
AU  - DelPizzo JJ
FAU - Vaughan, E Darracott Jr
AU  - Vaughan ED Jr
FAU - Sosa, R Ernest
AU  - Sosa RE
FAU - Fine, Ronnie G
AU  - Fine RG
FAU - Akhtar, Mohamed
AU  - Akhtar M
FAU - Scherr, Douglas S
AU  - Scherr DS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20061201
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Needle/standards
MH  - Carcinoma, Renal Cell/*pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*standards
MH  - Kidney/*pathology
MH  - Kidney Neoplasms/*pathology
MH  - Male
MH  - Middle Aged
MH  - Nephrectomy/methods
MH  - Sensitivity and Specificity
EDAT- 2006/11/10 09:00
MHDA- 2007/03/23 09:00
CRDT- 2006/11/10 09:00
PHST- 2006/11/10 09:00 [pubmed]
PHST- 2007/03/23 09:00 [medline]
PHST- 2006/11/10 09:00 [entrez]
AID - BJU6607 [pii]
AID - 10.1111/j.1464-410X.2006.06607.x [doi]
PST - ppublish
SO  - BJU Int. 2007 Feb;99(2):290-5. doi: 10.1111/j.1464-410X.2006.06607.x. Epub 2006 
      Dec 1.