PMID- 17093168
OWN - NLM
STAT- MEDLINE
DCOM- 20061214
LR  - 20230318
IS  - 0002-9165 (Print)
IS  - 0002-9165 (Linking)
VI  - 84
IP  - 5
DP  - 2006 Nov
TI  - Synergistic effect of supplemental enteral nutrients and exogenous glucagon-like 
      peptide 2 on intestinal adaptation in a rat model of short bowel syndrome.
PG  - 1142-50
AB  - BACKGROUND: Short bowel syndrome (SBS) can lead to intestinal failure and require 
      total or supplemental parenteral nutrition (TPN or PN, respectively). 
      Glucagon-like peptide 2 (GLP-2) is a nutrient-dependent, proglucagon-derived gut 
      hormone that stimulates intestinal adaptation. OBJECTIVE: Our objective was to 
      determine whether supplemental enteral nutrients (SEN) modulate the 
      intestinotrophic response to a low dose of GLP-2 coinfused with PN in a rat model 
      of SBS (60% jejunoileal resection plus cecectomy). DESIGN: Rats were randomly 
      assigned to 8 treatments by using a 2 x 2 x 2 factorial design and maintained 
      with either TPN or PN for 7 d. The 3 main treatment effects were the following: 
      transection or resection (TPN alone), +/- SEN (days 4-6), and +/- GLP-2 (100 mug 
      . kg body wt(-1) . d(-1)). RESULTS: The treatments induced differential growth of 
      duodenal and jejunal mucosa. Significant differences in villus height, crypt 
      depth, dry mass, and concentrations of protein and DNA were observed between the 
      treatments and TPN alone (SEN: 15-59% increase; GLP-2: 14-84% increase; and SEN + 
      GLP-2: 63-160% increase). Plasma concentrations of bioactive GLP-2 were 
      significantly greater with GLP-2 infusion (TPN alone: 25 +/- 9 pmol/L; SEN: 29 
      +/- 10 pmol/L; GLP-2: 59 +/- 31 pmol/L; SEN + GLP-2: 246 +/- 40 pmol/L) and 
      correlated with mucosal growth. Jejunal sucrase activity (in U/cm) was 
      significantly greater with SEN than without SEN. SEN + GLP-2 induced dramatic 
      mucosal growth and greater plasma concentration of GLP-2 (SEN x GLP-2 
      interaction, P < 0.0001). Resection significantly increased expression of 
      proglucagon mRNA in colon. CONCLUSIONS: Combination treatment with SEN and GLP-2 
      induced a synergistic response resulting in greater mucosal cellularity and 
      digestive capacity in parenterally fed rats with SBS. This shows that SEN improve 
      the intestinotrophic response to exogenous GLP-2, possibly by stimulating 
      enterocyte proliferation and differentiation.
FAU - Liu, Xiaowen
AU  - Liu X
AD  - Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, WI, 
      USA.
FAU - Nelson, David W
AU  - Nelson DW
FAU - Holst, Jens J
AU  - Holst JJ
FAU - Ney, Denise M
AU  - Ney DM
LA  - eng
GR  - R01-42835/PHS HHS/United States
GR  - T32-07665/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Clin Nutr
JT  - The American journal of clinical nutrition
JID - 0376027
RN  - 0 (Glucagon-Like Peptide 2)
RN  - 0 (RNA, Messenger)
RN  - EC 3.2.1.48 (Sucrase)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Animals
MH  - Cell Division/drug effects
MH  - Disease Models, Animal
MH  - Drug Synergism
MH  - Drug Therapy, Combination
MH  - *Enteral Nutrition
MH  - Glucagon-Like Peptide 2/*pharmacology
MH  - Intestinal Mucosa/*drug effects/growth & development
MH  - Intestine, Small/drug effects/pathology/surgery
MH  - Male
MH  - *Parenteral Nutrition, Total
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Short Bowel Syndrome/*therapy
MH  - Sucrase/metabolism
MH  - Treatment Outcome
EDAT- 2006/11/10 09:00
MHDA- 2006/12/15 09:00
CRDT- 2006/11/10 09:00
PHST- 2006/11/10 09:00 [pubmed]
PHST- 2006/12/15 09:00 [medline]
PHST- 2006/11/10 09:00 [entrez]
AID - S0002-9165(23)29161-3 [pii]
AID - 10.1093/ajcn/84.5.1142 [doi]
PST - ppublish
SO  - Am J Clin Nutr. 2006 Nov;84(5):1142-50. doi: 10.1093/ajcn/84.5.1142.