PMID- 17093498
OWN - NLM
STAT- MEDLINE
DCOM- 20070126
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 25
IP  - 23
DP  - 2006 Nov 29
TI  - Expression of the self-marker CD47 on dendritic cells governs their trafficking 
      to secondary lymphoid organs.
PG  - 5560-8
AB  - Dendritic cells (DCs) capture and process Ag in the periphery. Thus, traffic 
      through lymphatic vessels is mandatory before DCs relocate to lymph nodes where 
      they are dedicated to T-cell priming. Here, we show that the ubiquitous 
      self-marker CD47 selectively regulates DC, but not T and B cell trafficking 
      across lymphatic vessels and endothelial barriers in vivo. We find an altered 
      skin DC migration and impaired T-cell priming in CD47-deficient mice at steady 
      state and under inflammatory conditions. Competitive DC migration assays and 
      active immunization with myeloid DCs demonstrate that CD47 expression is required 
      on DCs but not on the endothelium for efficient DC trafficking and T-cell 
      responses. This migratory defect correlates with the quasi-disappearance of 
      splenic marginal zone DCs in nonmanipulated CD47-deficient mice. Nonetheless, 
      CCR7 expression and CCL19-driven chemotaxis remain intact. Our data reveal that 
      CD47 on DCs is a critical factor in controlling migration and efficient 
      initiation of the immune response.
FAU - Van, Vu Quang
AU  - Van VQ
AD  - Immunoregulation Laboratory, CHUM Research Center, University of Montreal, 
      Montreal, Quebec, Canada.
FAU - Lesage, Sylvie
AU  - Lesage S
FAU - Bouguermouh, Salim
AU  - Bouguermouh S
FAU - Gautier, Patrick
AU  - Gautier P
FAU - Rubio, Manuel
AU  - Rubio M
FAU - Levesque, Martin
AU  - Levesque M
FAU - Nguyen, Sebastien
AU  - Nguyen S
FAU - Galibert, Laurent
AU  - Galibert L
FAU - Sarfati, Marika
AU  - Sarfati M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061109
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (CD47 Antigen)
SB  - IM
MH  - Animals
MH  - CD47 Antigen/genetics/metabolism/*physiology
MH  - Cell Movement
MH  - *Chemotaxis
MH  - Dendritic Cells/chemistry/*immunology
MH  - Lymphatic Vessels/immunology
MH  - Lymphoid Tissue/immunology
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Skin/immunology
MH  - Spleen/*immunology
MH  - T-Lymphocytes/immunology
PMC - PMC1679770
EDAT- 2006/11/10 09:00
MHDA- 2007/01/27 09:00
PMCR- 2007/11/29
CRDT- 2006/11/10 09:00
PHST- 2006/05/11 00:00 [received]
PHST- 2006/10/10 00:00 [accepted]
PHST- 2006/11/10 09:00 [pubmed]
PHST- 2007/01/27 09:00 [medline]
PHST- 2006/11/10 09:00 [entrez]
PHST- 2007/11/29 00:00 [pmc-release]
AID - 7601415 [pii]
AID - 10.1038/sj.emboj.7601415 [doi]
PST - ppublish
SO  - EMBO J. 2006 Nov 29;25(23):5560-8. doi: 10.1038/sj.emboj.7601415. Epub 2006 Nov 
      9.