PMID- 17094896
OWN - NLM
STAT- MEDLINE
DCOM- 20071214
LR  - 20171116
VI  - 25
IP  - 11
DP  - 2006 Nov
TI  - [Antitumor effects of cocultured dendritic cells and cytokine-induced killer 
      cells on lung cancer in vitro and in vivo].
PG  - 1329-33
AB  - BACKGROUND & OBJECTIVE: Cytokine-induced killer (CIK) cells have high cytotoxic 
      activity against tumor cells. Dendritic cells (DCs) are the strongest 
      antigen-presenting cells (APC) and could increase the cytotoxic activity of 
      immunologic effector cells against tumor cells. This study was to investigate the 
      changes of phenotype, proliferative activity, and in vitro and in vivo 
      cytotoxicity of CIK cells after in vitro co-culturing with DCs. METHODS: DCs and 
      CIK cells were prepared routinely from human peripheral blood mononuclear cells 
      (PBMC), then CIK cells were cocultured with autologous DCs for 5 days at a 
      stimulator-to-responder ratio of 1:10 to prepare immunologic effector DC-CIK 
      cells. Phenotypes of DC-CIK cells were analyzed by flow cytometry; The in vitro 
      cytotoxicity of DC-CIK cells was detected by 3H-TdR incorporation method; the in 
      vivo antitumor activity was evaluated in BALB/c nude mice bearing A549 lung 
      cancer. RESULTS: At the 14th day of culture, compared with CIK cells, DC-CIK 
      cells got a significant increase of proliferation rate [(17.0+/-1.8) times vs. 
      (10.9+/-2.0) times, P<0.05] and CD3+CD56+ expression rate [(36.0+/-4.2)% vs. 
      (25.7+/-2.9)%, P<0.05], and resulted in an enhancement of cytotoxicity to A549 
      cells (P<0.05) in vivo. At the 51st day after inoculation of tumor cells, the 
      inhibition rate was significantly higher in DC-CIK group than in CIK group (62.9% 
      vs. 41.5%, P<0.05). DC-CIK cells and CIK cells showed significant inhibitory 
      effects on the growth of transplanted tumor cells as compared with control group 
      (P<0.01). CONCLUSION: DC-CIK cells have higher proliferative activity and 
      cytotoxicity in vitro and in vivo against lung cancer in comparison with CIK 
      cells alone.
FAU - Yang, Xin-Jing
AU  - Yang XJ
AD  - Department of Respiratory Medicine, The First Affiliated Hospital, Soochow 
      University, Suzhou, Jiangsu, 215006, P. R. China.
FAU - Huang, Jian-An
AU  - Huang JA
FAU - Lei, Wei
AU  - Lei W
FAU - Zhu, Yi-Bei
AU  - Zhu YB
FAU - Zhang, Xue-Guang
AU  - Zhang XG
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
RN  - 0 (CD3 Complex)
RN  - 0 (CD56 Antigen)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - CD3 Complex/metabolism
MH  - CD56 Antigen/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Cytokines/pharmacology
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/cytology/*immunology
MH  - Female
MH  - Humans
MH  - Immunotherapy, Adoptive
MH  - Killer Cells, Natural/*immunology
MH  - Lung Neoplasms/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Phenotype
EDAT- 2006/11/11 09:00
MHDA- 2007/12/15 09:00
CRDT- 2006/11/11 09:00
PHST- 2006/11/11 09:00 [pubmed]
PHST- 2007/12/15 09:00 [medline]
PHST- 2006/11/11 09:00 [entrez]
AID - 1000-467X2006111329 [pii]
PST - ppublish
SO  - Ai Zheng. 2006 Nov;25(11):1329-33.