PMID- 17094978
OWN - NLM
STAT- MEDLINE
DCOM- 20070125
LR  - 20131121
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 87
IP  - 1
DP  - 2007 Jan
TI  - Mifepristone alters expression of endometrial steroid receptors and their 
      cofactors in new users of medroxyprogesterone acetate.
PG  - 8-23
AB  - OBJECTIVE: To evaluate the effect of mifepristone on the expression of 
      endometrial steroid receptors and their co-factors in depot medroxyprogesterone 
      acetate (DMPA) users. DESIGN: A prospective, randomized, placebo-controlled 
      trial. SETTING: Reproductive research center. PATIENT(S): Fifty healthy women 
      with regular menstrual cycle. INTERVENTION(S): One hundred fifty milligrams of 
      DMPA were given every 3 months. Two pills (25 mg each) of placebo or mifepristone 
      were administered every 14 days during the DMPA therapy. Four endometrial biopsy 
      specimens were obtained from each patient. MAIN OUTCOME MEASURE(S): The 
      expression of estrogen receptor subtypes alpha and beta (ERalpha and ERbeta), 
      progesterone receptors A and B (PRAB and PRB), and androgen receptor messenger 
      RNA and protein was detected by real-time polymerase chain reaction and 
      immunohistochemistry, respectively. Steroid receptor coactivator 1 (SRC-1), 
      silencing mediator for retinoid and thyroid-hormone receptors, and cell 
      proliferation were evaluated by immunohistochemistry. RESULT(S): The expression 
      of endometrial ERalpha, PRAB, PRB, and SRC-1 was increased significantly after 1 
      week of mifepristone, but the increase was no longer seen after 10 weeks. A 
      positive correlation between endometrial ERalpha, PRAB, PRB, and SRC-1 production 
      and proliferation was demonstrated. CONCLUSION(S): Short-term exposure of 
      mifepristone in new starters of DMPA increases the expression of endometrial 
      ERalpha, PRAB, PRB, and SRC-1 and promotes cell proliferation. Prolonged exposure 
      to mifepristone does not alter the suppression of these receptors that are caused 
      by DMPA and continues to result in endometrial atrophy.
FAU - Jain, John K
AU  - Jain JK
AD  - Department of Obstetrics and Gynecology, The Keck School of Medicine, University 
      of Southern California, Los Angeles, California 90033, USA. jjain@usc.edu
FAU - Li, Aimin
AU  - Li A
FAU - Yang, Wangrong
AU  - Yang W
FAU - Minoo, Parviz
AU  - Minoo P
FAU - Felix, Juan C
AU  - Felix JC
LA  - eng
GR  - 1 R03 HDO 47322/PHS HHS/United States
GR  - R0-1 HD 43189/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
DEP - 20061107
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Contraceptives, Oral, Synthetic)
RN  - 0 (Drug Combinations)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Steroid)
RN  - 320T6RNW1F (Mifepristone)
RN  - C2QI4IOI2G (Medroxyprogesterone Acetate)
SB  - IM
MH  - Contraceptives, Oral, Synthetic/administration & dosage
MH  - Double-Blind Method
MH  - Drug Combinations
MH  - Endometrium/drug effects/*metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Medroxyprogesterone Acetate/*administration & dosage
MH  - Mifepristone/*administration & dosage
MH  - Placebo Effect
MH  - Protein Isoforms/metabolism
MH  - Receptors, Steroid/*metabolism
EDAT- 2006/11/11 09:00
MHDA- 2007/01/26 09:00
CRDT- 2006/11/11 09:00
PHST- 2006/02/15 00:00 [received]
PHST- 2006/05/22 00:00 [revised]
PHST- 2006/05/22 00:00 [accepted]
PHST- 2006/11/11 09:00 [pubmed]
PHST- 2007/01/26 09:00 [medline]
PHST- 2006/11/11 09:00 [entrez]
AID - S0015-0282(06)03171-2 [pii]
AID - 10.1016/j.fertnstert.2006.05.076 [doi]
PST - ppublish
SO  - Fertil Steril. 2007 Jan;87(1):8-23. doi: 10.1016/j.fertnstert.2006.05.076. Epub 
      2006 Nov 7.