PMID- 17095647
OWN - NLM
STAT- MEDLINE
DCOM- 20070411
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 292
IP  - 3
DP  - 2007 Mar
TI  - Monocyte chemoattractant protein-1 influences trauma-hemorrhage-induced distal 
      organ damage via regulation of keratinocyte-derived chemokine production.
PG  - R1110-6
AB  - Leukocyte infiltration, mediated by chemokines, is a key step in the development 
      of organ dysfunction. Lung and liver neutrophil infiltration following 
      trauma-hemorrhage is associated with upregulation of monocyte chemoattractant 
      protein-1 (MCP-1). Because MCP-1 is not a major attractant for neutrophils, we 
      hypothesized that MCP-1 influences neutrophil infiltration via regulation of 
      keratinocyte-derived chemokines (KC). To study this, male C3H/HeN mice were 
      pretreated with MCP-1 antiserum or control serum and subjected to 
      trauma-hemorrhage or sham operation. Animals were killed 4 h after resuscitation. 
      One group of trauma-hemorrhage mice receiving MCP-1 antiserum was also treated 
      with murine KC during resuscitation. Plasma levels and tissue content of MCP-1 
      and KC were determined by cytometric bead arrays. Immunohistochemistry was 
      performed to determine neutrophil infiltration; organ damage was assessed by 
      edema formation. Treatment with MCP-1 antiserum significantly decreased systemic, 
      lung, and liver levels of MCP-1 and KC following trauma-hemorrhage. This decrease 
      in MCP-1 levels was associated with decreased neutrophil infiltration and edema 
      formation in lung and liver following trauma-hemorrhage. Restitution of KC in 
      mice treated with MCP-1 antiserum restored tissue neutrophil infiltration and 
      edema. These results lead us to conclude that increased levels of MCP-1 cause 
      neutrophil accumulation and distant organ damage by regulating KC production 
      during the postinjury inflammatory response.
FAU - Frink, Michael
AU  - Frink M
AD  - Center for Surgical Research and Department of Surgery, University of Alabama at 
      Birmingham, Volker Hall-Suite G094, 1670 University Blvd., Birmingham, AL 
      35294-0019, USA.
FAU - Lu, Ailing
AU  - Lu A
FAU - Thobe, Bjoern M
AU  - Thobe BM
FAU - Hsieh, Ya-Ching
AU  - Hsieh YC
FAU - Choudhry, Mashkoor A
AU  - Choudhry MA
FAU - Schwacha, Martin G
AU  - Schwacha MG
FAU - Kunkel, Steven L
AU  - Kunkel SL
FAU - Chaudry, Irshad H
AU  - Chaudry IH
LA  - eng
GR  - K02 AI 49960/AI/NIAID NIH HHS/United States
GR  - R01 GM 37127/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061109
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/blood/*pharmacology
MH  - Chemokines/*biosynthesis/blood/genetics
MH  - Edema
MH  - Gene Expression Regulation/drug effects
MH  - Hemorrhage/*immunology/pathology
MH  - Immunohistochemistry
MH  - Keratinocytes/*drug effects/metabolism
MH  - Liver/drug effects/pathology
MH  - Lung/drug effects/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Neutrophil Infiltration/drug effects/immunology
MH  - Time Factors
MH  - Wounds and Injuries/immunology/*metabolism/pathology
EDAT- 2006/11/11 09:00
MHDA- 2007/04/12 09:00
CRDT- 2006/11/11 09:00
PHST- 2006/11/11 09:00 [pubmed]
PHST- 2007/04/12 09:00 [medline]
PHST- 2006/11/11 09:00 [entrez]
AID - 00650.2006 [pii]
AID - 10.1152/ajpregu.00650.2006 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2007 Mar;292(3):R1110-6. doi: 
      10.1152/ajpregu.00650.2006. Epub 2006 Nov 9.