PMID- 17096419
OWN - NLM
STAT- MEDLINE
DCOM- 20070306
LR  - 20131121
IS  - 1097-0134 (Electronic)
IS  - 0887-3585 (Linking)
VI  - 66
IP  - 2
DP  - 2007 Feb 1
TI  - NMR structure and binding studies confirm that PA4608 from Pseudomonas aeruginosa 
      is a PilZ domain and a c-di-GMP binding protein.
PG  - 266-71
AB  - PA4608 is a 125 residue protein from Pseudomonas aeruginosa with a recent 
      identification as a PilZ domain and putative bis-(3'-5')-cyclic dimeric guanosine 
      monophosphate (c-di-GMP) adaptor protein that plays a role in bacterial 
      second-messenger regulated processes. The nuclear magnetic resonance (NMR) 
      structure of PA4608 has been determined and c-di-GMP binding has been confirmed 
      by NMR titration studies. The monomeric core structure of PA4608 contains a 
      six-stranded anti-parallel beta barrel flanked by three helices. Conserved 
      surface residues among PA4608 homologs suggest the c-di-GMP binding site is at 
      one end of the barrel and includes residues in the helices as well as in the 
      unstructured N-terminus. Chemical shift changes in PA4608 resonances upon 
      titration with c-di-GMP confirm binding. This evidence supports the hypothesis 
      that proteins containing PilZ domains are the long-sought c-di-GMP adaptor 
      proteins.
CI  - Copyright 2006 Wiley-Liss, Inc.
FAU - Ramelot, Theresa A
AU  - Ramelot TA
AD  - Pacific Northwest National Laboratory, Biological Sciences Division, Richland, 
      Washington 99354, USA.
FAU - Yee, Adelinda
AU  - Yee A
FAU - Cort, John R
AU  - Cort JR
FAU - Semesi, Anthony
AU  - Semesi A
FAU - Arrowsmith, Cheryl H
AU  - Arrowsmith CH
FAU - Kennedy, Michael A
AU  - Kennedy MA
LA  - eng
GR  - P50-GM62413/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proteins
JT  - Proteins
JID - 8700181
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Bacterial Proteins)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*chemistry/metabolism
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/*chemistry/metabolism
MH  - Binding Sites
MH  - Cyclic GMP/*analogs & derivatives/metabolism
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Protein Binding
MH  - Protein Conformation
MH  - Protein Structure, Tertiary
MH  - Pseudomonas aeruginosa/*chemistry
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Vibrio cholerae/chemistry
EDAT- 2006/11/11 09:00
MHDA- 2007/03/07 09:00
CRDT- 2006/11/11 09:00
PHST- 2006/11/11 09:00 [pubmed]
PHST- 2007/03/07 09:00 [medline]
PHST- 2006/11/11 09:00 [entrez]
AID - 10.1002/prot.21199 [doi]
PST - ppublish
SO  - Proteins. 2007 Feb 1;66(2):266-71. doi: 10.1002/prot.21199.