PMID- 17097777
OWN - NLM
STAT- MEDLINE
DCOM- 20070719
LR  - 20181201
IS  - 0266-4356 (Print)
IS  - 0266-4356 (Linking)
VI  - 45
IP  - 4
DP  - 2007 Jun
TI  - Engineering of human tracheal tissue with collagen-enforced poly-lactic-glycolic 
      acid non-woven mesh: a preliminary study in nude mice.
PG  - 272-8
AB  - The purpose of the current study is to fabricate tissue engineered trachea with 
      poly-lactic-glycolic acid (PLGA) non-woven mesh enforced by collagen type I. PLGA 
      fibres coated with collagen solution were put together and fabricated into the 
      shape of a human trachea, after drying and cross-linking treatment, a non-woven 
      mesh with "C" shape formed. Chondrocytes from sheep nasal septum cartilage were 
      expanded in vitro and seeded into PLGA/collagen non-woven mesh in the density of 
      5.0 x 10(7)mL(-1). After 5 days of in vitro incubation, six Cell-PLGA/collagen 
      composites were implanted subcutaneously into the back of 6 nude mice to 
      prefabricate a tissue engineering trachea. Eight weeks later, the cartilage 
      formation was observed by gross inspection and histological examination. 
      Cartilage-like tissue in the shape of the initial PLGA/collagen scaffold had been 
      regenerated successfully without obvious inflammatory response. The tissue 
      engineered trachea cartilage consisted of evenly spaced lacunae embedded in 
      matrix stained red with safranin-O staining. The amount of GAGs in tissue 
      engineered trachea cartilage reached 71.42% of normal value in native cartilage. 
      This study demonstrated that collagen-enforced PLGA non-woven mesh facilitated 
      the adhesion and proliferation of chondrocytes, it also owned adequate mechanical 
      strength to serve as an ideal scaffold for trachea tissue engineering without 
      internal support.
FAU - Wu, Wei
AU  - Wu W
AD  - Department of Oral and Maxillofacial Surgery, Qindu Hospital, The Fourth Military 
      Medical University, Xi'an, PR China.
FAU - Feng, Xue
AU  - Feng X
FAU - Mao, Tianqiu
AU  - Mao T
FAU - Feng, Xinghua
AU  - Feng X
FAU - Ouyang, Hong-Wei
AU  - Ouyang HW
FAU - Zhao, Guifang
AU  - Zhao G
FAU - Chen, Fulin
AU  - Chen F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061113
PL  - Scotland
TA  - Br J Oral Maxillofac Surg
JT  - The British journal of oral & maxillofacial surgery
JID - 8405235
RN  - 0 (Biocompatible Materials)
RN  - 0 (Collagen Type I)
RN  - 0 (Coloring Agents)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Phenazines)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - XTX0YXU2HV (safranine T)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials/chemistry
MH  - Cartilage/anatomy & histology
MH  - Cell Adhesion
MH  - Cell Count
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chondrocytes/cytology
MH  - Chondrogenesis/physiology
MH  - *Collagen Type I/chemistry
MH  - Coloring Agents
MH  - Cross-Linking Reagents/chemistry
MH  - Desiccation
MH  - Glycosaminoglycans/analysis
MH  - Humans
MH  - *Lactic Acid/chemistry
MH  - Mice
MH  - Mice, Nude
MH  - Nasal Septum/cytology
MH  - Phenazines
MH  - *Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers/chemistry
MH  - Sheep
MH  - Subcutaneous Tissue/surgery
MH  - *Surgical Mesh
MH  - *Tissue Engineering/methods
MH  - Trachea/*anatomy & histology
EDAT- 2006/11/14 09:00
MHDA- 2007/07/20 09:00
CRDT- 2006/11/14 09:00
PHST- 2006/09/14 00:00 [accepted]
PHST- 2006/11/14 09:00 [pubmed]
PHST- 2007/07/20 09:00 [medline]
PHST- 2006/11/14 09:00 [entrez]
AID - S0266-4356(06)00174-4 [pii]
AID - 10.1016/j.bjoms.2006.09.004 [doi]
PST - ppublish
SO  - Br J Oral Maxillofac Surg. 2007 Jun;45(4):272-8. doi: 
      10.1016/j.bjoms.2006.09.004. Epub 2006 Nov 13.