PMID- 17097800
OWN - NLM
STAT- MEDLINE
DCOM- 20070927
LR  - 20220321
IS  - 0302-2838 (Print)
IS  - 0302-2838 (Linking)
VI  - 52
IP  - 2
DP  - 2007 Aug
TI  - Upregulation of penile brain-derived neurotrophic factor (BDNF) and activation of 
      the JAK/STAT signalling pathway in the major pelvic ganglion of the rat after 
      cavernous nerve transection.
PG  - 574-80
AB  - OBJECTIVES: Increasing attention is being focused on identifying novel approaches 
      to recover cavernous nerve (CN) function after injury or secondary to disease 
      states such as diabetes mellitus. We examined penile brain-derived neurotrophic 
      factor (BDNF) and neurotrophin-3 (NT-3) expression, and activation of the Janus 
      kinase (JAK)/signal transducer and activator of transcription (STAT) molecular 
      pathway in the major pelvic ganglion (MPG) after CN injury in the rat. METHODS: 
      Five groups of eight male Sprague-Dawley rats (4 mo, 250-300 g) were used in this 
      study. The penis and MPG with attached CN segment were harvested at 0 h 
      (controls), 12, and 24 h, as well as at 5 and 12 d after CN axotomy, for protein, 
      messenger RNA (mRNA), and immunohistochemical analysis. RESULTS: mRNA and protein 
      expression of BDNF was upregulated in the penis after injury (p<0.05). Levels of 
      NT-3 were unchanged. The JAK/STAT pathway was activated in the MPG after 
      transection, as evidenced by increased STAT1 (peak: 24 h) and STAT3 (peak: 5 d) 
      phosphorylation (p<0.01 vs. controls). CONCLUSIONS: This study demonstrates 
      increased expression of penile BDNF and upregulation of phosphorylated STAT1 and 
      STAT3 in the MPG in response to CN transection. Activation of the JAK/STAT 
      pathway after injury represents a promising new molecular target for modulating 
      CN survival and regeneration.
FAU - Bella, Anthony J
AU  - Bella AJ
AD  - Knuppe Molecular Urology Laboratory, Department of Urology, University of 
      California, San Francisco, CA 94143-0738, USA. abella@urology.ucsf.edu
FAU - Lin, Guiting
AU  - Lin G
FAU - Garcia, Maurice M
AU  - Garcia MM
FAU - Tantiwongse, Kavirach
AU  - Tantiwongse K
FAU - Brant, William O
AU  - Brant WO
FAU - Lin, Ching-Shwun
AU  - Lin CS
FAU - Lue, Tom F
AU  - Lue TF
LA  - eng
GR  - R37-DK-45370/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061030
PL  - Switzerland
TA  - Eur Urol
JT  - European urology
JID - 7512719
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (STAT Transcription Factors)
RN  - EC 2.7.10.2 (Jak1 protein, rat)
RN  - EC 2.7.10.2 (Janus Kinase 1)
RN  - EC 2.7.10.2 (Janus Kinases)
SB  - IM
CIN - Eur Urol. 2007 Aug;52(2):580-1. PMID: 17674438
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Ganglia, Parasympathetic/*metabolism
MH  - Immunoenzyme Techniques
MH  - Janus Kinase 1
MH  - Janus Kinases/*metabolism
MH  - Male
MH  - Penis/*innervation/*metabolism/surgery
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - STAT Transcription Factors/*metabolism
MH  - Signal Transduction
MH  - Up-Regulation
EDAT- 2006/11/14 09:00
MHDA- 2007/09/28 09:00
CRDT- 2006/11/14 09:00
PHST- 2006/07/26 00:00 [received]
PHST- 2006/10/18 00:00 [accepted]
PHST- 2006/11/14 09:00 [pubmed]
PHST- 2007/09/28 09:00 [medline]
PHST- 2006/11/14 09:00 [entrez]
AID - S0302-2838(06)01334-0 [pii]
AID - 10.1016/j.eururo.2006.10.043 [doi]
PST - ppublish
SO  - Eur Urol. 2007 Aug;52(2):574-80. doi: 10.1016/j.eururo.2006.10.043. Epub 2006 Oct 
      30.