PMID- 1709812
OWN - NLM
STAT- MEDLINE
DCOM- 19910628
LR  - 20190609
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1064
IP  - 2
DP  - 1991 May 7
TI  - Formation of ion channels in planar lipid bilayer membranes by synthetic basic 
      peptides.
PG  - 256-66
AB  - We made use of a planar lipid bilayer system to examine the action of synthetic 
      basic peptides which model the prepiece moiety of mitochondrial protein 
      precursors and have antibacterial activity against Gram-positive bacteria. The 
      sequences of the peptides used were as follows: Ac-(Ala-Arg-Leu)3-NHCH3 (3(3], 
      Ac-(Leu-Ala-Arg-Leu)2-NHCH3 (4(2], Ac-(Leu-Ala-Arg-Leu)3-NHCH3 (4(3], 
      Ac-(Leu-Leu-Ala-Arg-Leu)2-NHCH3 (5(2]. These peptides interacted differently with 
      planar lipid bilayer membranes and membrane conductance increased by the 
      formation of ion channels. The effects of the peptides on the macroscopic 
      current-increase and on the probability of channel formation, at the single 
      channel level were in the order of 4(3) greater than 4(2) approximately 5(2) much 
      greater than 3(3), a finding which correlates with the antibacterial activity of 
      these peptides. The micromolar (microM) order concentration at which the channel 
      was formed resembles that causing antibacterial activity. Thus, the peptide 
      antibacterial activity may occur through an increase in ion permeability of the 
      bacterial membrane. The single-channel properties were investigated in detail 
      using 4(3), the peptide with the highest ion channel-forming activity. Many types 
      of channels were observed with respect to conductance (2-750 pS) and voltage 
      dependency of gating. However, the channels were all cation-selective. These 
      results suggest that the ion channels formed by peptide 4(3) may be able to take 
      on a variety of conformations and/or assembly.
FAU - Anzai, K
AU  - Anzai K
AD  - Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
FAU - Hamasuna, M
AU  - Hamasuna M
FAU - Kadono, H
AU  - Kadono H
FAU - Lee, S
AU  - Lee S
FAU - Aoyagi, H
AU  - Aoyagi H
FAU - Kirino, Y
AU  - Kirino Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Ion Channels)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anti-Bacterial Agents/chemistry/*pharmacology
MH  - Biological Transport
MH  - Cell Membrane/drug effects
MH  - Ion Channels/chemistry/drug effects/*metabolism
MH  - *Lipid Bilayers
MH  - Membrane Potentials
MH  - Molecular Sequence Data
MH  - Peptides/chemistry/*pharmacology
EDAT- 1991/05/07 00:00
MHDA- 1991/05/07 00:01
CRDT- 1991/05/07 00:00
PHST- 1991/05/07 00:00 [pubmed]
PHST- 1991/05/07 00:01 [medline]
PHST- 1991/05/07 00:00 [entrez]
AID - 0005-2736(91)90310-5 [pii]
AID - 10.1016/0005-2736(91)90310-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1991 May 7;1064(2):256-66. doi: 
      10.1016/0005-2736(91)90310-5.