PMID- 17098388
OWN - NLM
STAT- MEDLINE
DCOM- 20071018
LR  - 20191210
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 171
IP  - 1
DP  - 2007 Aug 24
TI  - Determination of bromadiolone in whole blood by high-performance liquid 
      chromatography coupled with electrospray ionization tandem mass spectrometry.
PG  - 52-6
AB  - A rapid, sensitive and selective high-performance liquid chromatography tandem 
      mass spectrometric method (HPLC/MS-MS) has been developed and validated for the 
      determination of bromadiolone in whole blood using warfarin as an internal 
      standard (IS). Bromadiolone was extracted from the whole blood samples by 
      liquid-liquid extraction with ethyl acetate. Multiple-reaction monitoring (MRM) 
      was used to detect bromadiolone and IS, using precursor --> product ion 
      combinations at m/z 527 --> 465 and 307 --> 161, respectively. The calibration 
      curve was linear (r2=0.998) in the concentration range of 0.5-100.0 ng/mL with a 
      lower limit of quantification of 0.5 ng/mL in whole blood. Intra- and inter-day 
      relative standard deviations (R.S.D.s) were less than 7.5 and 11.9%, 
      respectively. Recoveries of bromadiolone ranged from 82.1 to 85.2%. This method 
      is found to be determined trace bromadiolone in whole blood and can be used in 
      the diagnosis of the poisoned human beings.
FAU - Jin, Mi-Cong
AU  - Jin MC
AD  - Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
FAU - Ren, Yi-Ping
AU  - Ren YP
FAU - Xu, Xiao-Min
AU  - Xu XM
FAU - Chen, Xiao-Hong
AU  - Chen XH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20061113
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (4-Hydroxycoumarins)
RN  - 0 (Rodenticides)
RN  - J2FR050NM5 (bromadiolone)
SB  - IM
MH  - 4-Hydroxycoumarins/*blood/chemistry
MH  - Female
MH  - Forensic Toxicology/*methods
MH  - *Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Middle Aged
MH  - Molecular Structure
MH  - Poisoning/diagnosis
MH  - Rodenticides/*blood/chemistry
MH  - *Spectrometry, Mass, Electrospray Ionization
EDAT- 2006/11/14 09:00
MHDA- 2007/10/19 09:00
CRDT- 2006/11/14 09:00
PHST- 2006/06/04 00:00 [received]
PHST- 2006/09/26 00:00 [revised]
PHST- 2006/10/08 00:00 [accepted]
PHST- 2006/11/14 09:00 [pubmed]
PHST- 2007/10/19 09:00 [medline]
PHST- 2006/11/14 09:00 [entrez]
AID - S0379-0738(06)00619-0 [pii]
AID - 10.1016/j.forsciint.2006.10.005 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2007 Aug 24;171(1):52-6. doi: 10.1016/j.forsciint.2006.10.005. 
      Epub 2006 Nov 13.