PMID- 17098977
OWN - NLM
STAT- MEDLINE
DCOM- 20061219
LR  - 20201006
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 87
IP  - Pt 12
DP  - 2006 Dec
TI  - MCP-1, a highly expressed chemokine in dengue haemorrhagic fever/dengue shock 
      syndrome patients, may cause permeability change, possibly through reduced tight 
      junctions of vascular endothelium cells.
PG  - 3623-3630
LID - 10.1099/vir.0.82093-0 [doi]
AB  - Vascular leakage, one hallmark of dengue haemorrhagic fever (DHF) and dengue 
      shock syndrome, has been linked to the mediators secreted from cells in the 
      circulatory system. In this study, extremely high expression levels of monocyte 
      chemoattractant protein-1 (MCP-1) were found in the plasma of DHF patients 
      compared with low MCP-1 expression levels in the plasma of enterovirus 
      71-infected patients. It was also found that MCP-1 expression was induced in 
      dengue virus 2 (DV2)-infected monocytes and lymphocytes, but not in liver or 
      endothelial cells. Exposing monolayers of human umbilical vein endothelial cells 
      (HUVECs) to recombinant human MCP-1 (rhMCP-1) or to the culture supernatant of 
      DV2-infected human monocytes increased the vascular permeability of the cells. 
      MCP-1-neutralizing monoclonal antibody only partially prevented monolayer 
      permeability change. Consistently, the distribution of the tight junction protein 
      ZO-1 on the cellular membranes of HUVECs was disrupted by rhMCP-1 or by the 
      conditioned medium of DV2-infected monocytes. In summary, it was found that the 
      increased permeability and disrupted tight junctions of human vascular 
      endothelium cells were effected through a mechanism partially dependent on MCP-1, 
      which was secreted by DV2-infected monocytes and lymphocytes.
FAU - Lee, Ying-Ray
AU  - Lee YR
AD  - Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung 
      University, 1 Da-Shue Road, Tainan 701, Taiwan.
FAU - Liu, Ming-Tao
AU  - Liu MT
AD  - Tainan Hospital, Department of Health, Executive Yuan, Tainan, Taiwan.
FAU - Lei, Huan-Yao
AU  - Lei HY
AD  - Department of Microbiology and Immunology, College of Medicine, National Cheng 
      Kung University, 1 Da-Shue Road, Tainan 701, Taiwan.
AD  - Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung 
      University, 1 Da-Shue Road, Tainan 701, Taiwan.
FAU - Liu, Ching-Chuan
AU  - Liu CC
AD  - Department of Pediatrics, College of Medicine, National Cheng Kung University, 1 
      Da-Shue Road, Tainan 701, Taiwan.
FAU - Wu, Jing-Ming
AU  - Wu JM
AD  - Department of Pediatrics, College of Medicine, National Cheng Kung University, 1 
      Da-Shue Road, Tainan 701, Taiwan.
FAU - Tung, Yi-Ching
AU  - Tung YC
AD  - Department of Clinical Laboratory, Kaohsiung Medical University, Kaohsiung City, 
      Taiwan.
FAU - Lin, Yee-Shin
AU  - Lin YS
AD  - Department of Microbiology and Immunology, College of Medicine, National Cheng 
      Kung University, 1 Da-Shue Road, Tainan 701, Taiwan.
FAU - Yeh, Trai-Ming
AU  - Yeh TM
AD  - Department of Microbiology and Immunology, College of Medicine, National Cheng 
      Kung University, 1 Da-Shue Road, Tainan 701, Taiwan.
FAU - Chen, Shun-Hua
AU  - Chen SH
AD  - Department of Microbiology and Immunology, College of Medicine, National Cheng 
      Kung University, 1 Da-Shue Road, Tainan 701, Taiwan.
FAU - Liu, Hsiao-Sheng
AU  - Liu HS
AD  - Department of Microbiology and Immunology, College of Medicine, National Cheng 
      Kung University, 1 Da-Shue Road, Tainan 701, Taiwan.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TJP1 protein, human)
RN  - 0 (Zonula Occludens-1 Protein)
SB  - IM
MH  - *Capillary Permeability
MH  - Cell Line
MH  - Cell Membrane/chemistry
MH  - Chemokine CCL2/antagonists & inhibitors/blood/genetics/*physiology
MH  - Culture Media, Conditioned
MH  - Endothelial Cells/metabolism/virology
MH  - Enterovirus Infections/immunology/physiopathology
MH  - Gene Expression
MH  - Hepatocytes/metabolism/virology
MH  - Humans
MH  - Lymphocytes/metabolism/virology
MH  - Membrane Proteins/analysis
MH  - Microscopy, Fluorescence
MH  - Monocytes/metabolism/virology
MH  - Phosphoproteins/analysis
MH  - RNA, Messenger/analysis
MH  - Recombinant Proteins/pharmacology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Severe Dengue/immunology/pathology/*physiopathology
MH  - Tight Junctions/physiology/ultrastructure
MH  - Up-Regulation
MH  - Zonula Occludens-1 Protein
EDAT- 2006/11/14 09:00
MHDA- 2006/12/21 09:00
CRDT- 2006/11/14 09:00
PHST- 2006/11/14 09:00 [pubmed]
PHST- 2006/12/21 09:00 [medline]
PHST- 2006/11/14 09:00 [entrez]
AID - 10.1099/vir.0.82093-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2006 Dec;87(Pt 12):3623-3630. doi: 10.1099/vir.0.82093-0.