PMID- 17100834
OWN - NLM
STAT- MEDLINE
DCOM- 20070119
LR  - 20061114
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 24
IP  - 9
DP  - 2006 Nov
TI  - Developmental changes of neurotrophin mRNA expression in the layers of rat visual 
      cortex.
PG  - 2453-60
AB  - Neurotrophins are essential factors for the structural, neurochemical and 
      functional maturation of the brain including developmental and adult plasticity. 
      Northern blots and polymerase chain reaction revealed the expression of 
      neurotrophin 4 (NT4), neurotrophin 3 (NT3), brain-derived neurotrophic factor 
      (BDNF) and nerve growth factor (NGF) in the cortex. The cellular producers of NT3 
      and BDNF have been characterized by anatomical methods as being mostly pyramidal, 
      and the tyrosine kinase B (TrkB) receptor is expressed by many cortical neurons. 
      However, these methods have so far failed to identify the cells producing NT4 and 
      NGF mRNA. These factors are much lower in expression than, e.g. BDNF, and 
      apparently remain below detection levels of in situ hybridization. Given their 
      specific actions on cell types and afferent systems, knowledge about the 
      producing cell types is highly desirable. To narrow down on the producing cell 
      types, we quantified by reverse transcriptase-polymerase chain reaction (RT-PCR) 
      the developmental changes of BDNF, NT3, NT4, NGF and TrkB mRNA expression in 
      total visual cortex lysates, and in the cortical layers dissected by tangential 
      cryostat sectioning. We found dramatic changes in laminar expression of NT3 and 
      NGF, mild changes of NT4, and no changes of BDNF and TrkB mRNA. For instance, NT3 
      is important early on for thalamocortical axons, and we found transient peaks of 
      NT3 mRNA expression first in layer VI, then in layer IV. NT4 mRNA was in layers 
      IV and VI, suggesting NT4 protein production in thalamorecipient layers, but peak 
      expression gradually shifted to upper layers as did NGF expression. The 
      layer-specific developmental expression shifts of neurotrophin mRNAs correlate 
      with morphogenetic processes.
FAU - Patz, Silke
AU  - Patz S
AD  - AG Entwicklungsneurobiologie, Fakultat fur Biologie, ND 6/72, Ruhr-Universitat, 
      44780 Bochum, Germany. silke.patz@rub.de
FAU - Wahle, Petra
AU  - Wahle P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Nerve Growth Factors/*biosynthesis
MH  - Neurons/*cytology/*metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Visual Cortex/*cytology/growth & development/*metabolism
EDAT- 2006/11/15 09:00
MHDA- 2007/01/20 09:00
CRDT- 2006/11/15 09:00
PHST- 2006/11/15 09:00 [pubmed]
PHST- 2007/01/20 09:00 [medline]
PHST- 2006/11/15 09:00 [entrez]
AID - EJN5126 [pii]
AID - 10.1111/j.1460-9568.2006.05126.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2006 Nov;24(9):2453-60. doi: 10.1111/j.1460-9568.2006.05126.x.