PMID- 17101147
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20141120
IS  - 0022-2828 (Print)
IS  - 0022-2828 (Linking)
VI  - 42
IP  - 1
DP  - 2007 Jan
TI  - Transplantation of mesenchymal stem cells attenuates myocardial injury and 
      dysfunction in a rat model of acute myocarditis.
PG  - 88-97
AB  - Acute myocarditis is a non-ischemic inflammatory disease of the myocardium for 
      which there is currently no specific treatment. We have previously shown that 
      mesenchymal stem cells (MSC) can ameliorate heart injury during acute ischemia 
      and in dilated cardiomyopathy; however, the therapeutic potential in acute 
      myocarditis is unclear. In this study, we investigated the ability of MSC to 
      attenuate myocardial injury and dysfunction during the acute phase of 
      experimental myocarditis. Ten-week-old male Lewis rats were injected with porcine 
      myosin to induce myocarditis. Cultured MSC (3x10(6) cells/rat) were injected 
      intravenously 7 days after myosin injection. At 3 weeks, myosin injection 
      resulted in severe inflammation and significant deterioration of cardiac 
      function. MSC transplantation attenuated increases in CD68-positive inflammatory 
      cells and monocyte chemoattractant protein-1 (MCP-1) expression in myocardium, 
      and improved cardiac function in this model. Furthermore, myocardial capillary 
      density was higher in myocarditis tissue, and was further increased by MSC 
      transplantation. In vitro, cultured adult rat cardiomyocytes were injured in 
      response to MCP-1, whereas this effect was attenuated by MSC-derived conditioned 
      medium, suggesting cardioprotective effects of MSC acting in a paracrine manner. 
      MSC transplantation attenuated myocardial injury and dysfunction in a rat model 
      of acute myocarditis, at least in part through paracrine effects of MSC.
FAU - Ohnishi, Shunsuke
AU  - Ohnishi S
AD  - Department of Regenerative Medicine and Tissue Engineering, National 
      Cardiovascular Center Research Institute, Fujishirodai 5-7-1, Osaka 565-8565, 
      Japan.
FAU - Yanagawa, Bobby
AU  - Yanagawa B
FAU - Tanaka, Koichi
AU  - Tanaka K
FAU - Miyahara, Yoshinori
AU  - Miyahara Y
FAU - Obata, Hiroaki
AU  - Obata H
FAU - Kataoka, Masaharu
AU  - Kataoka M
FAU - Kodama, Makoto
AU  - Kodama M
FAU - Ishibashi-Ueda, Hatsue
AU  - Ishibashi-Ueda H
FAU - Kangawa, Kenji
AU  - Kangawa K
FAU - Kitamura, Soichiro
AU  - Kitamura S
FAU - Nagaya, Noritoshi
AU  - Nagaya N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061113
PL  - England
TA  - J Mol Cell Cardiol
JT  - Journal of molecular and cellular cardiology
JID - 0262322
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Apoptosis
MH  - Creatine Kinase/metabolism
MH  - Disease Models, Animal
MH  - In Vitro Techniques
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Myocarditis/pathology/physiopathology/*therapy
MH  - Myocytes, Cardiac/pathology
MH  - Neovascularization, Physiologic
MH  - Rats
MH  - Rats, Inbred Lew
EDAT- 2006/11/15 09:00
MHDA- 2007/02/28 09:00
CRDT- 2006/11/15 09:00
PHST- 2006/05/11 00:00 [received]
PHST- 2006/08/29 00:00 [revised]
PHST- 2006/10/02 00:00 [accepted]
PHST- 2006/11/15 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2006/11/15 09:00 [entrez]
AID - S0022-2828(06)00959-X [pii]
AID - 10.1016/j.yjmcc.2006.10.003 [doi]
PST - ppublish
SO  - J Mol Cell Cardiol. 2007 Jan;42(1):88-97. doi: 10.1016/j.yjmcc.2006.10.003. Epub 
      2006 Nov 13.