PMID- 17102917
OWN - NLM
STAT- MEDLINE
DCOM- 20070406
LR  - 20181113
IS  - 0364-2313 (Print)
IS  - 0364-2313 (Linking)
VI  - 30
IP  - 12
DP  - 2006 Dec
TI  - Effect of glucocorticoid pretreatment on oxidative liver injury and survival in 
      jaundiced rats with endotoxin cholangitis.
PG  - 2217-26
AB  - INTRODUCTION: Biliary tract infection is associated with high mortality. This 
      study investigated the effect of glucocorticoid pretreatment on 
      lipopolysaccharide (LPS)-induced cholangitis. METHODS: Rats undergoing either 
      sham operation or ligation of the extrahepatic bile duct (BDL) for 2 weeks were 
      randomly assigned to receive intravenous injections of dexamethasone (DX) or 
      normal saline (NS) prior to infusing LPS into the biliary tract. The plasma 
      levels of tumor necrosis factor-alpha (TNFalpha), chemokines monocyte 
      chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 (MIP-2) 
      as well as liver mRNA expression of MCP-1 and MIP-2 were determined. Infiltration 
      of monocytes, Kupffer cells, and neutrophils in rat liver were studied with 
      immunohistochemistry. Oxidative liver injury was measured by the malondialdehyde 
      (MDA) content. RESULTS: Dexamethasone pretreatment resulted in significantly 
      decreased plasma levels of TNFalpha at 1 hour, MCP-1 and MIP-2 at 2 and 3 hours, 
      and decreased liver MCP-1 mRNA expression at 3 hours following LPS infusion in 
      BDL-DX rats than in BDL-NS rats. The number of inflammatory cells in the liver 
      was significantly different between sham- and BDL-treated rats but was not 
      affected by DX pretreatment. Pretreatment with DX resulted in significantly 
      decreased liver MDA contents in the BDL-DX group than that in the BDL-NS group. 
      Jaundiced rats pretreated with 5 mg DX prior to infusion of 1 g of LPS were 6.8 
      times more likely to survive than those that were not pretreated. CONCLUSIONS: 
      Pretreatment of jaundiced, LPS-treated rats with a supraphysiological dose of 
      dexamethasone may rescue their lives by suppression of chemokine expression and 
      alleviation of oxidative liver injury.
FAU - Lee, Chi Wei
AU  - Lee CW
AD  - Department of Emergency Medicine, E-Da Hospital, I-Shou University, 1 E-Da Road, 
      Jiau-shu Tsuen, Yan-chau Shiang, 824, Kaohsiung, Taiwan.
FAU - Chuang, Jiin Haur
AU  - Chuang JH
FAU - Wang, Pei Wen
AU  - Wang PW
FAU - Chang, Nyuk Kong
AU  - Chang NK
FAU - Wang, Hsiu Chuan
AU  - Wang HC
FAU - Huang, Chao Cheng
AU  - Huang CC
FAU - Tiao, Mao Meng
AU  - Tiao MM
FAU - Lo, Sing Kai
AU  - Lo SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Surg
JT  - World journal of surgery
JID - 7704052
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Glucocorticoids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Monokines)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CXCL2
MH  - Cholangitis/complications/*metabolism/pathology
MH  - Dexamethasone/*therapeutic use
MH  - Glucocorticoids/*therapeutic use
MH  - Jaundice/etiology/*mortality
MH  - Lipopolysaccharides/administration & dosage
MH  - Liver/*drug effects/metabolism
MH  - Male
MH  - Monokines/biosynthesis
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Survival Rate
EDAT- 2006/11/15 09:00
MHDA- 2007/04/07 09:00
CRDT- 2006/11/15 09:00
PHST- 2006/11/15 09:00 [pubmed]
PHST- 2007/04/07 09:00 [medline]
PHST- 2006/11/15 09:00 [entrez]
AID - 10.1007/s00268-006-0143-0 [doi]
PST - ppublish
SO  - World J Surg. 2006 Dec;30(12):2217-26. doi: 10.1007/s00268-006-0143-0.