PMID- 17103220
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20181113
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 116
IP  - 2
DP  - 2007 Apr
TI  - Probing the W chromosome of the codling moth, Cydia pomonella, with sequences 
      from microdissected sex chromatin.
PG  - 135-45
AB  - The W chromosome of the codling moth, Cydia pomonella, like that of most 
      Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin 
      body in somatic cells. We collected chromatin samples by laser microdissection 
      from euchromatin and W-chromatin bodies. DNA from the samples was amplified by 
      degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to 
      prepare painting probes and start an analysis of the W-chromosome sequence 
      composition. With fluorescence in situ hybridization (FISH), the euchromatin 
      probe labelled all chromosomes, whereas the W-chromatin DNA proved to be a highly 
      specific W-chromosome painting probe. For sequence analysis, DOP-PCR-generated 
      DNA fragments were cloned, sequenced, and tested by Southern hybridization. We 
      recovered single-copy and low-copy W-specific sequences, a sequence that was 
      located only in the W and the Z chromosome, multi-copy sequences that were 
      enriched in the W chromosome but occurred also elsewhere, and ubiquitous 
      multi-copy sequences. Three of the multi-copy sequences were recognized as 
      derived from hitherto unknown retrotransposons. The results show that our 
      approach is feasible and that the W-chromosome composition of C. pomonella is not 
      principally different from that of Bombyx mori or from that of Y chromosomes of 
      several species with an XY sex-determining mechanism. The W chromosome has 
      attracted repetitive sequences during evolution but also contains unique 
      sequences.
FAU - Fukova, Iva
AU  - Fukova I
AD  - Institute of Entomology, Biology Centre, ASCR, Branisovska 31, 370 05 Ceske 
      Budejovice, Czech Republic.
FAU - Traut, Walther
AU  - Traut W
FAU - Vitkova, Magda
AU  - Vitkova M
FAU - Nguyen, Petr
AU  - Nguyen P
FAU - Kubickova, Svatava
AU  - Kubickova S
FAU - Marec, Frantisek
AU  - Marec F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061114
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA Primers)
RN  - 0 (Molecular Probes)
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Painting/methods
MH  - DNA Primers
MH  - In Situ Hybridization, Fluorescence
MH  - Microdissection
MH  - Molecular Probes/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Moths/*genetics
MH  - Oligonucleotides
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sex Chromatin/genetics/*metabolism/ultrastructure
MH  - Sex Chromosomes/*genetics
MH  - Species Specificity
EDAT- 2006/11/15 09:00
MHDA- 2008/01/09 09:00
CRDT- 2006/11/15 09:00
PHST- 2006/08/07 00:00 [received]
PHST- 2006/10/04 00:00 [accepted]
PHST- 2006/10/02 00:00 [revised]
PHST- 2006/11/15 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2006/11/15 09:00 [entrez]
AID - 10.1007/s00412-006-0086-0 [doi]
PST - ppublish
SO  - Chromosoma. 2007 Apr;116(2):135-45. doi: 10.1007/s00412-006-0086-0. Epub 2006 Nov 
      14.