PMID- 17105151
OWN - NLM
STAT- MEDLINE
DCOM- 20070213
LR  - 20131121
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 78
IP  - 22
DP  - 2006 Nov 15
TI  - In vivo investigation of the distribution and the local speciation of selenium in 
      Allium cepa L. by means of microscopic X-ray absorption near-edge structure 
      spectroscopy and confocal microscopic X-ray fluorescence analysis.
PG  - 7616-24
AB  - In this work, microscopic X-ray absorption near-edge structure spectroscopy 
      (mu-XANES) and confocal microscopic X-ray fluorescence analysis (mu-XRF) were 
      used for the in vivo determination of the distribution of total selenium and for 
      the local speciation of selenium in roots and leaves of onion. Selected Allium 
      cepa L. plants were grown hydroponically in a standard medium containing 
      inorganic selenium compounds (selenite or selenate). The measurements were 
      performed in vivo, that is, on living plants without the need for any form of 
      sampling or sample pretreatment and without the necessity for cutting plant 
      tissues into pieces. Distinct energy differences of the XANES spectra of various 
      selenium reference compounds having different oxidation states allow adjusting 
      the excitation energies used for mu-XRF mapping in such a manner that the 
      distribution of selenium in various oxidation states is obtained with a spatial 
      resolution of a few tens of micrometers within the virtual cross section of the 
      onion tissues. We find that the ratio of inorganic to amino acid selenium 
      compounds differs in various subparts of the plant. Detailed in vivo 
      investigation of the distribution of various selenium species in virtual cross 
      sections of root tips and green leaf shows that the selenium transport takes 
      place via different mechanisms, depending on the nature of the selenium compounds 
      originally taken up.
FAU - Bulska, Ewa
AU  - Bulska E
AD  - Faculty of Chemistry, Warsaw University, Warsaw, Poland. ebulska@ chem.uw.edu.pl
FAU - Wysocka, Irena A
AU  - Wysocka IA
FAU - Wierzbicka, Ma Lstrok Gorzata H
AU  - Wierzbicka ML
FAU - Proost, Kristof
AU  - Proost K
FAU - Janssens, Koen
AU  - Janssens K
FAU - Falkenberg, Gerald
AU  - Falkenberg G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Amino Acids)
RN  - 0 (Organoselenium Compounds)
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Amino Acids/analysis
MH  - Microscopy, Confocal/methods
MH  - Microscopy, Fluorescence/methods
MH  - Onions/*chemistry
MH  - Organoselenium Compounds/*analysis
MH  - Oxidation-Reduction
MH  - Plant Leaves/chemistry/ultrastructure
MH  - Plant Roots/chemistry/ultrastructure
MH  - Selenium/*analysis
MH  - Spectrometry, X-Ray Emission/*methods
EDAT- 2006/11/16 09:00
MHDA- 2007/02/14 09:00
CRDT- 2006/11/16 09:00
PHST- 2006/11/16 09:00 [pubmed]
PHST- 2007/02/14 09:00 [medline]
PHST- 2006/11/16 09:00 [entrez]
AID - 10.1021/ac060380s [doi]
PST - ppublish
SO  - Anal Chem. 2006 Nov 15;78(22):7616-24. doi: 10.1021/ac060380s.