PMID- 17105783
OWN - NLM
STAT- MEDLINE
DCOM- 20070606
LR  - 20220129
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 13
IP  - 1
DP  - 2007 Jan
TI  - Expression and regulation of prostaglandin E synthase isoforms in human 
      myometrium with labour.
PG  - 69-75
AB  - Since the controversies regarding the use of non-steroidal anti-inflammatory 
      drugs (NSAIDs) and selective cyclo-oxygenase (COX)-2 antagonists for the 
      treatment of preterm labour (PTL), more emphasis has been placed on investigating 
      the terminal synthases involved in the production of prostaglandins (PGs) to 
      allow more targeted therapy in PTL. Prostaglandin E(2) (PGE(2)) is synthesized by 
      one of three enzymes, cytosolic prostaglandin E synthase (cPGES), microsomal 
      PGES-1 (mPGES-1) and microsomal PGES-2 (mPGES-2). We have determined (i) the 
      immuno-localization of all three PGES enzymes in lower segment pregnant human 
      myometrium, (ii) the expression of PGES and COX-2 mRNA expression at term and 
      preterm gestation with and without labour and (iii) the effect of interleukin 
      (IL)-1beta on COX-2 and PGES mRNA and protein expression in human myometrial 
      smooth muscle (HMSM) cell cultures. We show mPGES-1 protein located predominantly 
      in myometrial and vascular smooth muscle cells (SMCs), whilst mPGES-2 protein is 
      largely in stromal cells surrounding the SMC and cPGES is diffusely located 
      throughout the myometrium. Expression of mPGES-2 mRNA increased with term labour 
      and PTL and expression of COX-2 and mPGES-1 mRNA with term labour, whereas cPGES 
      expression did not change. IL-1beta stimulated release of PGE(2) by HMSM cells 
      and increased COX-2 and mPGES-1 mRNA and protein expression. Thus, COX-2 
      expression and mPGES-1 expression are co-ordinately up-regulated in lower segment 
      myometrium with term labour and with IL-1beta treatment in HMSM cells.
FAU - Astle, S
AU  - Astle S
AD  - Clinical Sciences Research Institute, Warwick Medical School, UHCW Trust, 
      Coventry, UK.
FAU - Newton, R
AU  - Newton R
FAU - Thornton, S
AU  - Thornton S
FAU - Vatish, M
AU  - Vatish M
FAU - Slater, D M
AU  - Slater DM
LA  - eng
GR  - G0400347/MRC_/Medical Research Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061114
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Interleukin-1beta)
RN  - 0 (Isoenzymes)
RN  - 0 (Membrane Proteins)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.99.3 (PTGES protein, human)
RN  - EC 5.3.99.3 (PTGES2 protein, human)
RN  - EC 5.3.99.3 (Prostaglandin-E Synthases)
SB  - IM
MH  - Cells, Cultured
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Female
MH  - *Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interleukin-1beta/pharmacology
MH  - Intramolecular Oxidoreductases/*genetics/*metabolism
MH  - Isoenzymes/genetics/metabolism
MH  - Labor, Obstetric/*genetics/*metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - Myometrium/*metabolism
MH  - Pregnancy
MH  - Prostaglandin-E Synthases
EDAT- 2006/11/16 09:00
MHDA- 2007/06/07 09:00
CRDT- 2006/11/16 09:00
PHST- 2006/11/16 09:00 [pubmed]
PHST- 2007/06/07 09:00 [medline]
PHST- 2006/11/16 09:00 [entrez]
AID - gal093 [pii]
AID - 10.1093/molehr/gal093 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2007 Jan;13(1):69-75. doi: 10.1093/molehr/gal093. Epub 2006 Nov 
      14.