PMID- 17105815
OWN - NLM
STAT- MEDLINE
DCOM- 20070402
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 6
DP  - 2007 Mar 15
TI  - Donor-derived cells and human graft-versus-host disease of the skin.
PG  - 2663-5
AB  - Graft-versus-host disease (GvHD)-induced apoptosis of the skin targets both 
      epidermal keratinocytes and dermal endothelial cells. We studied the 
      donor-versus-recipient origin of GvHD of these target cells in skin of 18 
      sex-mismatched hematopoietic stem-cell transplant (HSCT) recipients. Combining XY 
      fluorescence in situ hybridization (FISH) and double immunostaining, and further 
      3D tissue Z-stack analysis, we found keratinocytes and endothelial cells of donor 
      origin, but only in patients with GvHD. Using terminal dUTP nick-end labeling 
      (TUNEL) assay on sister sections, we found a correlation between the numbers of 
      chimeric and apoptotic epidermal and endothelial cells. Moreover, donor-derived 
      cells were more numerous and preferentially distributed in the areas of severe 
      GvHD damage in biopsies performed early in the course of GvHD, whereas they were 
      less numerous and found in the whole epidermis in late biopsies. Because 
      donor-derived cells were found at the site and at the time of maximum tissue 
      damage, they could contribute to epidermal and microvessel repair.
FAU - Murata, Hideyuki
AU  - Murata H
AD  - Institut National de la Sante et de la Recherche Medicale (INSERM), U728, Paris, 
      France.
FAU - Janin, Anne
AU  - Janin A
FAU - Leboeuf, Christophe
AU  - Leboeuf C
FAU - Soulier, Jean
AU  - Soulier J
FAU - Gluckman, Eliane
AU  - Gluckman E
FAU - Meignin, Veronique
AU  - Meignin V
FAU - Socie, Gerard
AU  - Socie G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061114
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Biopsy
MH  - Bone Marrow Transplantation/adverse effects
MH  - Chimerism
MH  - Female
MH  - Graft vs Host Disease/*immunology/*pathology
MH  - Humans
MH  - Male
MH  - Skin Diseases/*immunology/*pathology/surgery
MH  - *Tissue Donors
MH  - Transplantation, Homologous
EDAT- 2006/11/16 09:00
MHDA- 2007/04/03 09:00
CRDT- 2006/11/16 09:00
PHST- 2006/11/16 09:00 [pubmed]
PHST- 2007/04/03 09:00 [medline]
PHST- 2006/11/16 09:00 [entrez]
AID - S0006-4971(20)41884-1 [pii]
AID - 10.1182/blood-2006-07-033902 [doi]
PST - ppublish
SO  - Blood. 2007 Mar 15;109(6):2663-5. doi: 10.1182/blood-2006-07-033902. Epub 2006 
      Nov 14.