PMID- 17107614
OWN - NLM
STAT- MEDLINE
DCOM- 20061215
LR  - 20220330
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 3
DP  - 2006 Nov 15
TI  - Serendipitous identification of natural intergenotypic recombinants of hepatitis 
      C in Ireland.
PG  - 95
AB  - BACKGROUND: Recombination between hepatitis C single stranded RNA viruses is a 
      rare event. Natural viable intragenotypic and intergenotypic recombinants between 
      1b-1a, 1a-1c and 2k-1b, 2i-6p, respectively, have been reported. Diagnostically 
      recombinants represent an intriguing challenge. Hepatitis C genotype is defined 
      by interrogation of the sequence composition of the 5' untranslated region 
      [5'UTR]. Occasionally, ambiguous specimens require further investigation of the 
      genome, usually by interrogation of the NS5B region. The original purpose of this 
      study was to confirm the existence of a suspected mixed genotype infection of 
      genotypes 2 and 4 by clonal analysis at the NS5B region of the genome in two 
      specimens from two separate individuals. This initial identification of genotype 
      was based on analysis of the 5'UTR of the genome by reverse line probe 
      hybridisation [RLPH]. RESULTS: The original diagnosis of a mixed genotype 
      infection was not confirmed by clonal analysis of the NS5B region of the genome. 
      The phylogenetic analysis indicated that both specimens were natural 
      intergenotypic recombinant forms of HCV. The recombination was between genotypes 
      2k and 1b for both specimens. The recombination break point was identified as 
      occurring within the NS2 region of the genome. CONCLUSION: The viral recombinants 
      identified here resemble the recombinant form originally identified in Russia. 
      The RLPH pattern observed in this study may be a signature indicative of this 
      particular type of intergenotype recombinant of hepatitis C meriting clonal 
      analysis of NS2.
FAU - Moreau, Isabelle
AU  - Moreau I
AD  - Molecular Virology Diagnostic & Research Laboratory, Department of Medicine, 
      Clinical Sciences Building, Cork University Hospital, Cork, Ireland. 
      i.moreau@ucc.ie
FAU - Hegarty, Susan
AU  - Hegarty S
FAU - Levis, John
AU  - Levis J
FAU - Sheehy, Patrick
AU  - Sheehy P
FAU - Crosbie, Orla
AU  - Crosbie O
FAU - Kenny-Walsh, Elizabeth
AU  - Kenny-Walsh E
FAU - Fanning, Liam J
AU  - Fanning LJ
LA  - eng
SI  - GENBANK/AB031663
SI  - GENBANK/AY587845
SI  - GENBANK/D50485
SI  - GENBANK/DQ417427
SI  - GENBANK/DQ417428
SI  - GENBANK/DQ417429
SI  - GENBANK/DQ417430
SI  - GENBANK/DQ417431
SI  - GENBANK/DQ417432
SI  - GENBANK/DQ417433
SI  - GENBANK/DQ417434
SI  - GENBANK/DQ417435
SI  - GENBANK/DQ417436
SI  - GENBANK/DQ417437
SI  - GENBANK/DQ417438
SI  - GENBANK/DQ417439
SI  - GENBANK/DQ417440
SI  - GENBANK/DQ417441
SI  - GENBANK/DQ417442
SI  - GENBANK/DQ417443
SI  - GENBANK/DQ417444
SI  - GENBANK/DQ417445
SI  - GENBANK/DQ417446
SI  - GENBANK/DQ417447
SI  - GENBANK/DQ417448
SI  - GENBANK/DQ417449
SI  - GENBANK/DQ417450
SI  - GENBANK/DQ417451
SI  - GENBANK/DQ417452
SI  - GENBANK/DQ839386
SI  - GENBANK/DQ839387
SI  - GENBANK/DQ839388
SI  - GENBANK/DQ839389
SI  - GENBANK/DQ839390
SI  - GENBANK/DQ904446
PT  - Journal Article
DEP - 20061115
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (HVR1 protein, Hepatitis C virus)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.48 (NS-5 protein, hepatitis C virus)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Genotype
MH  - Hepacivirus/classification/*genetics/isolation & purification
MH  - Hepatitis C/virology
MH  - Humans
MH  - Ireland
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - *Recombination, Genetic
MH  - Sequence Alignment
MH  - Sequence Analysis, Protein
MH  - Viral Nonstructural Proteins/chemistry/genetics
MH  - Viral Proteins/chemistry/genetics
PMC - PMC1654145
EDAT- 2006/11/17 09:00
MHDA- 2006/12/16 09:00
PMCR- 2006/11/15
CRDT- 2006/11/17 09:00
PHST- 2006/09/20 00:00 [received]
PHST- 2006/11/15 00:00 [accepted]
PHST- 2006/11/17 09:00 [pubmed]
PHST- 2006/12/16 09:00 [medline]
PHST- 2006/11/17 09:00 [entrez]
PHST- 2006/11/15 00:00 [pmc-release]
AID - 1743-422X-3-95 [pii]
AID - 10.1186/1743-422X-3-95 [doi]
PST - epublish
SO  - Virol J. 2006 Nov 15;3:95. doi: 10.1186/1743-422X-3-95.