PMID- 17109760
OWN - NLM
STAT- MEDLINE
DCOM- 20061204
LR  - 20181113
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 7
DP  - 2006 Nov 17
TI  - Construction of a 7-fold BAC library and cytogenetic mapping of 10 genes in the 
      giant panda (Ailuropoda melanoleuca).
PG  - 294
AB  - BACKGROUND: The giant panda, one of the most primitive carnivores, is an 
      endangered animal. Although it has been the subject of many interesting studies 
      during recent years, little is known about its genome. In order to promote 
      research on this genome, a bacterial artificial chromosome (BAC) library of the 
      giant panda was constructed in this study. RESULTS: This BAC library contains 
      198,844 clones with an average insert size of 108 kb, which represents 
      approximately seven equivalents of the giant panda haploid genome. Screening the 
      library with 15 genes and 8 microsatellite markers demonstrates that it is 
      representative and has good genome coverage. Furthermore, ten BAC clones 
      harbouring AGXT, GHR, FSHR, IRBP, SOX14, TTR, BDNF, NT-4, LH and ZFX1 were mapped 
      to 8 pairs of giant panda chromosomes by fluorescence in situ hybridization 
      (FISH). CONCLUSION: This is the first large-insert genomic DNA library for the 
      giant panda, and will contribute to understanding this endangered species in the 
      areas of genome sequencing, physical mapping, gene cloning and comparative 
      genomic studies. We also identified the physical locations of ten genes on their 
      relative chromosomes by FISH, providing a preliminary framework for further 
      development of a high resolution cytogenetic map of the giant panda.
FAU - Liu, Wei
AU  - Liu W
AD  - State Key Laboratory for Agro-Biotechnology, China Agricultural University, 
      Beijing 100094, China. weiliucn04@hotmail.com
FAU - Zhao, Yonghui
AU  - Zhao Y
FAU - Liu, Zhaoliang
AU  - Liu Z
FAU - Zhang, Ying
AU  - Zhang Y
FAU - Lian, Zhengxing
AU  - Lian Z
FAU - Li, Ning
AU  - Li N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061117
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
SB  - IM
MH  - Animals
MH  - Chromosome Mapping/*methods
MH  - *Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Mammalian/*genetics
MH  - *Gene Library
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microsatellite Repeats
MH  - Ursidae/*genetics
PMC - PMC1664575
EDAT- 2006/11/18 09:00
MHDA- 2006/12/09 09:00
PMCR- 2006/11/17
CRDT- 2006/11/18 09:00
PHST- 2006/08/01 00:00 [received]
PHST- 2006/11/17 00:00 [accepted]
PHST- 2006/11/18 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2006/11/18 09:00 [entrez]
PHST- 2006/11/17 00:00 [pmc-release]
AID - 1471-2164-7-294 [pii]
AID - 10.1186/1471-2164-7-294 [doi]
PST - epublish
SO  - BMC Genomics. 2006 Nov 17;7:294. doi: 10.1186/1471-2164-7-294.