PMID- 1711012
OWN - NLM
STAT- MEDLINE
DCOM- 19910724
LR  - 20121115
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 4
IP  - 1
DP  - 1991
TI  - Double-labeling in situ hybridization analysis of mRNAs for carbonic anhydrase II 
      and myelin basic protein: expression in developing cultured glial cells.
PG  - 1-10
AB  - We applied in situ hybridization to analyze the location and the developmental 
      changes in the distribution of the transcripts for carbonic anhydrase II (CAII) 
      and myelin basic protein (MBP) in mouse primary cultured glial cells. Both mRNAs 
      were localized to the oligodendrocyte using double-labeling in situ 
      hybridization. No evidence for CAII transcripts in astrocytes was obtained, 
      indicating that CAII is expressed only by oligodendrocytes in normal rodent glia. 
      As early as 48 h after plating, CAII and MBP mRNAs are present in a few, small 
      round cells. Message is present 2-4 days before levels of these proteins can be 
      detected in similar primary glial cultures. The intensity of labeling for MBP and 
      CAII mRNA positive cells increases significantly during the second week but then 
      decreases after the end of the third week. Only the oligodendrocyte perikaryon 
      and a few processes are positive during the first week. In contrast, at 14 days, 
      a large number of cell processes in addition to the cell bodies are heavily 
      stained for both mRNAs. Both mRNAs could be detected far away from the cell body, 
      up to 250 microns in some cell processes. Some segments on a cell process 
      accumulate higher levels of mRNA than other areas. These areas may correspond to 
      the accumulation of free ribosomes and to starting points for the membrane sheets 
      elaborated by cultured oligodendrocytes. The developmental profile for timing and 
      distribution of these two messages mimics closely their in situ pattern.
FAU - Ghandour, M S
AU  - Ghandour MS
AD  - Department of Anatomy and Cell Biology, Wayne State University, School of 
      Medicine, Detroit, Michigan 48201.
FAU - Skoff, R P
AU  - Skoff RP
LA  - eng
GR  - NS 15338/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Myelin Basic Protein)
RN  - 0 (RNA, Messenger)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Animals
MH  - Brain/cytology
MH  - Carbonic Anhydrases/*biosynthesis/genetics
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Mice
MH  - Myelin Basic Protein/*biosynthesis/genetics
MH  - *Nucleic Acid Hybridization
MH  - Oligodendroglia/cytology/*metabolism
MH  - RNA, Messenger/*analysis
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1002/glia.440040102 [doi]
PST - ppublish
SO  - Glia. 1991;4(1):1-10. doi: 10.1002/glia.440040102.