PMID- 17112039 OWN - NLM STAT- MEDLINE DCOM- 20100610 LR - 20171116 IS - 1000-0593 (Print) IS - 1000-0593 (Linking) VI - 26 IP - 9 DP - 2006 Sep TI - [The interaction of acridine orange with anionic surfactant and its application to the determination of protein]. PG - 1653-5 AB - The change in the UV-absorption spectrum and fluorescence spectrum of acridine orange(AO) due to the addition of surfactant dodecyi benzene sulfonic acid sodium sait (SDBS) and bovine serum albumin(BSA) was studied. Meanwhile, the effects of the in situ formed AOAO dimer in SDBS as a fluorescence probe and BSA were discussed. A new method for the determination of BSA using fluorescence is presented. The results indicate that the method is sensitive and rapid. The linear range of determination is 0-4. 17 x 10(-7) mol x L(-1). The relative standard deviation is 1.9%, and the detection limit is 8.73 x 10(-10) mol x L(-1). FAU - Shi, Yan AU - Shi Y AD - Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China. FAU - Zheng, Wei-Wan AU - Zheng WW FAU - Wu, Xiao-Xing AU - Wu XX FAU - Zhou, De-Hong AU - Zhou DH LA - chi PT - Journal Article PL - China TA - Guang Pu Xue Yu Guang Pu Fen Xi JT - Guang pu xue yu guang pu fen xi = Guang pu JID - 9424805 RN - 0 (Anions) RN - 0 (Benzenesulfonates) RN - 0 (Fluorescent Dyes) RN - 0 (Surface-Active Agents) RN - 27432CM55Q (Serum Albumin, Bovine) RN - 60NSK897G9 (dodecylbenzenesulfonic acid) RN - F30N4O6XVV (Acridine Orange) SB - IM MH - Acridine Orange/*chemistry MH - Animals MH - Anions MH - Benzenesulfonates/*chemistry MH - Cattle MH - *Fluorescence MH - Fluorescent Dyes/chemistry MH - Hydrogen-Ion Concentration MH - Reproducibility of Results MH - Serum Albumin, Bovine/analysis/*chemistry MH - Spectrometry, Fluorescence MH - Spectrophotometry, Ultraviolet MH - Surface-Active Agents/chemistry MH - Temperature EDAT- 2006/11/23 09:00 MHDA- 2010/06/11 06:00 CRDT- 2006/11/23 09:00 PHST- 2006/11/23 09:00 [pubmed] PHST- 2010/06/11 06:00 [medline] PHST- 2006/11/23 09:00 [entrez] PST - ppublish SO - Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Sep;26(9):1653-5.