PMID- 17113579
OWN - NLM
STAT- MEDLINE
DCOM- 20070405
LR  - 20161124
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 556
IP  - 1-3
DP  - 2007 Feb 5
TI  - Peroxisome proliferator-activated receptor alpha-independent effects of 
      peroxisome proliferators on cysteinyl leukotriene production in mast cells.
PG  - 172-80
AB  - The effects of peroxisome proliferators, the ligands of a nuclear receptor 
      peroxisome proliferator-activated receptor (PPAR) alpha, on cysteinyl leukotriene 
      production were investigated in rodent mast cells. Peroxisome proliferators 
      Wy-14,643 (30 microM) and fenofibrate (100 microM) significantly inhibited the 
      cysteinyl leukotriene production that was induced by antigen (Ag) treatment after 
      overnight sensitization to Ag specific immunoglobulin E (IgE) in a rat basophilic 
      leukemia (RBL)-2H3 mast cell line. Similar inhibition by these drugs was observed 
      in IgE and Ag-treated mouse bone marrow-derived mast cells, A23187-treated 
      RBL-2H3 and A23187-treated mouse peritoneal macrophages. Wy-14,643 (30 microM) 
      and fenofibrate (100 microM) did not affect the release of radioactivity from 
      RBL-2H3 pre-incubated with [(3)H]-arachidonic acid, which is considered an index 
      of phospholipase A(2) activity. Wy-14,643 (30 microM) and fenofibrate (100 
      microM) did not directly inhibit 5-lipoxygenase activity. Troglitazone was found 
      to directly inhibit the activity of 5-lipoxygenase. The PPARalpha mRNA level was 
      at less than the limit of detection for the realtime polymerase chain reaction 
      both in RBL-2H3 and bone marrow-derived mast cells. Wy-14,643 (30 microM) and 
      fenofibrate (100 microM) did not induce acyl-CoA oxidase mRNA in RBL-2H3, which 
      was reported to be induced by peroxisome proliferators via PPARalpha in 
      hepatocytes. Wy-14,643 (30 microM) and fenofibrate (100 microM) inhibited the 
      cysteinyl leukotriene production in bone marrow-derived mast cells from 
      PPARalpha-null mice. It was concluded that the inhibitory effects of these 
      peroxisome proliferators on cysteinyl leukotriene production are independent of 
      PPARalpha in mast cells.
FAU - Yamashita, Masamichi
AU  - Yamashita M
AD  - Department of Pathophysiology, Tohoku Pharmaceutical University, 4-4-1 
      Komatsushima, Aoba-ku, Sendai, Miyagi 981-8558, Japan. may@tohoku-pharm.ac.jp
LA  - eng
PT  - Journal Article
DEP - 20061019
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Leukotrienes)
RN  - 0 (PPAR alpha)
RN  - 0 (Peroxisome Proliferators)
RN  - 0 (Pyrimidines)
RN  - 0 (cysteinyl-leukotriene)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 86C4MRT55A (pirinixic acid)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
RN  - EC 1.3.3.6 (Acyl-CoA Oxidase)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.2.1.- (Hexosaminidases)
RN  - K848JZ4886 (Cysteine)
RN  - U202363UOS (Fenofibrate)
SB  - IM
MH  - Acyl-CoA Oxidase/metabolism
MH  - Animals
MH  - Arachidonate 5-Lipoxygenase/metabolism
MH  - Bone Marrow Cells/cytology/drug effects/metabolism
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Cysteine/*biosynthesis
MH  - Fenofibrate/*pharmacology
MH  - Hexosaminidases/metabolism
MH  - Immunoglobulin E/metabolism
MH  - Leukotrienes/*biosynthesis
MH  - Macrophages, Peritoneal/cytology/drug effects/metabolism
MH  - Mast Cells/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - PPAR alpha/genetics/*physiology
MH  - Peroxisome Proliferators/*pharmacology
MH  - Phospholipases A/metabolism
MH  - Pyrimidines/*pharmacology
MH  - Rats
EDAT- 2006/11/23 09:00
MHDA- 2007/04/06 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/05/30 00:00 [received]
PHST- 2006/10/06 00:00 [revised]
PHST- 2006/10/10 00:00 [accepted]
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/04/06 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - S0014-2999(06)01152-6 [pii]
AID - 10.1016/j.ejphar.2006.10.032 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2007 Feb 5;556(1-3):172-80. doi: 10.1016/j.ejphar.2006.10.032. 
      Epub 2006 Oct 19.