PMID- 17114432
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20210103
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 177
IP  - 11
DP  - 2006 Dec 1
TI  - Distinct and non-overlapping T cell receptor repertoires expanded by DNA 
      vaccination in wild-type and HER-2 transgenic BALB/c mice.
PG  - 7626-33
AB  - Central tolerance to tumor-associated Ags is an immune-escape mechanism that 
      significantly limits the TCR repertoires available for tumor eradication. The 
      repertoires expanded in wild-type BALB/c and rat-HER-2/neu (rHER-2) transgenic 
      BALB-neuT mice following DNA immunization against rHER-2 were compared by 
      spectratyping the variable (V)beta and the joining (J)beta CDR 3. Following 
      immunization, BALB/c mice raised a strong response. Every mouse used one or more 
      CD8+ T cell rearrangements of the Vbeta9-Jbeta1.2 segments characterized by 
      distinct length of the CDR3 and specific for 63-71 or 1206-1214 rHER-2 peptides. 
      In addition, two CD4+ T cell rearrangements recurred in >50% of mice. Instead, 
      BALB-neuT mice displayed a limited response to rHER-2. Their repertoire is 
      smaller and uses different rearrangements confined to CD4+ T cells. Thus, central 
      tolerance in BALB-neuT mice acts by silencing the BALB/c mice self-reactive 
      repertoire and reducing the size of the CD8+ T cell component. CD8+ and CD4+ T 
      cells from both wild-type and transgenic mice home to tumors. This definition of 
      the T cell repertoires available is critical to the designing of immunological 
      maneuvers able to elicit an effective immune reaction against HER-2-driven 
      carcinogenesis.
FAU - Rolla, Simona
AU  - Rolla S
AD  - Dipartimento di Scienze Cliniche e Biologiche, University of Turin, Turin, Italy.
FAU - Nicolo, Chiara
AU  - Nicolo C
FAU - Malinarich, Silvia
AU  - Malinarich S
FAU - Orsini, Massimiliano
AU  - Orsini M
FAU - Forni, Guido
AU  - Forni G
FAU - Cavallo, Federica
AU  - Cavallo F
FAU - Ria, Francesco
AU  - Ria F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, DNA)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Neoplasm/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Female
MH  - Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
MH  - Genes, T-Cell Receptor beta
MH  - *Immune Tolerance
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - RNA, Messenger/analysis
MH  - Receptor, ErbB-2/*immunology
MH  - Receptors, Antigen, T-Cell/*immunology
MH  - Recombinant Proteins/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transfection
MH  - *Tumor Escape
MH  - *Vaccines, DNA
EDAT- 2006/11/23 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - 177/11/7626 [pii]
AID - 10.4049/jimmunol.177.11.7626 [doi]
PST - ppublish
SO  - J Immunol. 2006 Dec 1;177(11):7626-33. doi: 10.4049/jimmunol.177.11.7626.