PMID- 17114449
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 177
IP  - 11
DP  - 2006 Dec 1
TI  - Characterization of myeloid and plasmacytoid dendritic cells in human lung.
PG  - 7784-93
AB  - Dendritic cells (DCs) are bone marrow-derived mononuclear cells that play a 
      central role in the initiation of immune responses. Because human lung DCs have 
      been incompletely characterized, we enumerated and phenotyped mononuclear cell 
      populations from excess lung tissue obtained at surgery. Myeloid DCs (MDCs) were 
      identified as CD1c(+)CD11c(+)CD14(-)HLA-DR(+) cells and comprised approximately 
      2% of low autofluorescent (LAF) mononuclear cells. Plasmacytoid DCs (PDCs) were 
      characterized as CD123(+)CD11c(-)CD14(-)HLA-DR(+) cells and comprised 
      approximately 1.0% of the LAF mononuclear cells. Cells enriched in MDCs expressed 
      CD86, moderate CD80, and little CD40, but cells enriched in PDCs had little to no 
      expression of these three costimulatory molecules. CD11c(+)CD14(-) 
      lineage-negative (MDC-enriched) LAF cells were isolated and shown to be much more 
      potent in stimulating an alloreaction than CD11c(+)CD14(+) lineage-negative 
      (monocyte-enriched) LAF cells. PDC-enriched cells were more capable of responding 
      to a TLR-7 agonist by secreting IFN-alpha than MDC-enriched cells. MDC-enriched 
      cells were either CD123(+) or CD123(-), but both subsets secreted cytokines and 
      chemokines typical of MDC upon stimulation with a TLR-4 agonist and both subsets 
      failed to secrete IFN-alpha upon stimulation with a TLR-7 agonist. By 
      immunohistochemistry, we identified MDCs throughout different anatomical 
      locations of the lung. However, our method did not allow the localization of PDCs 
      with certainty. In conclusion, in the human lung MDCs were twice as numerous and 
      expressed higher levels of costimulatory molecules than PDCs. Our data suggest 
      that both lung DC subsets exert distinct immune modulatory functions.
FAU - Masten, Barbara J
AU  - Masten BJ
AD  - Departments of Pathology, University of New Mexico, Albuquerque, NM 87131, USA.
FAU - Olson, Gwyneth K
AU  - Olson GK
FAU - Tarleton, Christy A
AU  - Tarleton CA
FAU - Rund, Chad
AU  - Rund C
FAU - Schuyler, Mark
AU  - Schuyler M
FAU - Mehran, Reza
AU  - Mehran R
FAU - Archibeque, Tereassa
AU  - Archibeque T
FAU - Lipscomb, Mary F
AU  - Lipscomb MF
LA  - eng
GR  - P50 HL 56134/HL/NHLBI NIH HHS/United States
GR  - U19 AI 5234/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Cell Lineage
MH  - Dendritic Cells/*cytology/*immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Leukocytes, Mononuclear/immunology
MH  - Lung/*cytology/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Myeloid Cells/*immunology
MH  - Phenotype
EDAT- 2006/11/23 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - 177/11/7784 [pii]
AID - 10.4049/jimmunol.177.11.7784 [doi]
PST - ppublish
SO  - J Immunol. 2006 Dec 1;177(11):7784-93. doi: 10.4049/jimmunol.177.11.7784.