PMID- 17114931
OWN - NLM
STAT- MEDLINE
DCOM- 20090715
LR  - 20190516
IS  - 1555-8584 (Electronic)
IS  - 1547-6286 (Linking)
VI  - 2
IP  - 4
DP  - 2005 Oct-Dec
TI  - Hypomodification of transfer RNA in cancer with respect to queuosine.
PG  - 143-8
AB  - Queuosine is a highly modified nucleoside analogue of guanosine. It is present 
      only in the first position of anticodon loop of specific tRNA i.e., tRNA(his), 
      tRNA(asp), tRNA(asn) and tRNA(tyr) and post transcriptionally modified with 
      base-for-base exchange of guanine to queuine. The transfer RNA modifying enzyme 
      transfer RNA guanine transglycosylase (TGTase) catalyzes the modification of 
      tRNAs. Transfer RNA is completely modified with respect to queuosine in mature 
      tissue, however modification is often incomplete in mitotically active cells. 
      Hypomodification of transfer RNA is correlated with cell proliferation and 
      malignancy. In the present study queuosine modification of transfer RNA and 
      TGTase activity is compared in normal, Dalton's lymphoma ascites transplanted 
      (DLAT) cancerous and queuine treated DLAT cancerous mouse liver. Transfer RNA of 
      cancerous mouse is hypomodified in terms of queuosine modification. TGTase 
      activity of cancerous mouse is found to decrease to less then half of enzyme 
      activity of normal mouse; suggesting that the enzyme may be responsible for 
      transfer RNA hypomodification. Exogenous treatment of queuine during development 
      of cancer improves the queuosine modification of transfer RNA. The activators 
      NaPP and ATP enhance TGTase activity of normal and DLAT cancerous mouse, where as 
      7mG inhibits the TGTase activity.
FAU - Pathak, Chandramani
AU  - Pathak C
AD  - School of Studies In Biochemistry, Jiwaji University, Gwalior, India.
FAU - Jaiswal, Yogesh K
AU  - Jaiswal YK
FAU - Vinayak, Manjula
AU  - Vinayak M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051205
PL  - United States
TA  - RNA Biol
JT  - RNA biology
JID - 101235328
RN  - 57072-36-3 (Nucleoside Q)
RN  - 9014-25-9 (RNA, Transfer)
SB  - IM
MH  - Animals
MH  - Female
MH  - Lymphoma/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred AKR
MH  - Nucleoside Q/*metabolism
MH  - RNA, Transfer/*metabolism
EDAT- 2006/11/23 09:00
MHDA- 2009/07/16 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2009/07/16 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - 2417 [pii]
AID - 10.4161/rna.2.4.2417 [doi]
PST - ppublish
SO  - RNA Biol. 2005 Oct-Dec;2(4):143-8. doi: 10.4161/rna.2.4.2417. Epub 2005 Dec 5.