PMID- 17115448
OWN - NLM
STAT- MEDLINE
DCOM- 20070717
LR  - 20131121
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 97
IP  - 4
DP  - 2007 Jul 1
TI  - Nitrifying bacterial communities in an aquaculture wastewater treatment system 
      using fluorescence in situ hybridization (FISH), 16S rRNA gene cloning, and 
      phylogenetic analysis.
PG  - 985-90
AB  - Aquaculture, especially shrimp farming, has played a major role in the growth of 
      Thailand's economy in recent years, as well as in many South East Asian 
      countries. However, the nutrient discharges from these activities have caused 
      adverse impacts on the quality of the receiving waterways. In particular 
      nitrogenous compounds, which may accumulate in aquaculture ponds, can be toxic to 
      aquatic animals and cause environmental problems such as eutrophication. The 
      mineralization process is well known, but certain aspects of the microbial 
      ecology of nitrifiers, the microorganisms that convert ammonia to nitrate, are 
      poorly understood. A previously reported enrichment of nitrifying bacteria 
      (ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB)) from a 
      shrimp farm inoculated in a sequencing batch reactor (SBR) was studied by 
      molecular methods. The initial identification and partial quantification of the 
      nitrifying bacteria (AOB and NOB) were carried out by fluorescence in situ 
      hybridization (FISH) using previously published 16S rRNA-targeting 
      oligonucleotide probes. The two dominant bacterial groups detected by FISH were 
      from the Cytophaga-Flavobacterium-Bacteroides and Proteobacteria (beta 
      subdivision) phyla. Published FISH probes for Nitrobacter and Nitrospira did not 
      hybridize to any of the bacterial cells. Therefore it is likely that new 
      communities of NOBs, differing from previously reported ones, exist in the 
      enrichments. Molecular genetic techniques (cloning, sequencing, and phylogenetic 
      analysis) targeting the 16S rRNA genes from the nitrifying enrichments were 
      performed to identify putative AOBs and NOBs.
CI  - (c) 2006 Wiley Periodicals, Inc.
FAU - Paungfoo, Chanyarat
AU  - Paungfoo C
AD  - Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of 
      Songkla University, Hat-Yai 90112, Thailand.
FAU - Prasertsan, Poonsuk
AU  - Prasertsan P
FAU - Burrell, Paul C
AU  - Burrell PC
FAU - Intrasungkha, Nugul
AU  - Intrasungkha N
FAU - Blackall, Linda L
AU  - Blackall LL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Animals
MH  - *Aquaculture
MH  - *Cloning, Molecular
MH  - *Ecosystem
MH  - *In Situ Hybridization, Fluorescence
MH  - Nitrogen/*metabolism
MH  - Penaeidae
MH  - *Phylogeny
MH  - Proteobacteria/classification/genetics/*metabolism
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Waste Disposal, Fluid/*methods
EDAT- 2006/11/23 09:00
MHDA- 2007/07/18 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/07/18 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - 10.1002/bit.21270 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2007 Jul 1;97(4):985-90. doi: 10.1002/bit.21270.