PMID- 17117131
OWN - NLM
STAT- MEDLINE
DCOM- 20070117
LR  - 20131121
IS  - 1073-2322 (Print)
IS  - 1073-2322 (Linking)
VI  - 26
IP  - 6
DP  - 2006 Dec
TI  - Sex dimorphisms in activated mesenchymal stem cell function.
PG  - 571-4
AB  - The plasticity of bone marrow-derived stem cells (BMSCs) has resulted in positive 
      remodeling and the regeneration of viable tissues. However, BMSC release of 
      growth factors, which limit apoptosis and inflammation, may play an important 
      role in conferring organ protection. Recent studies also indicate that those 
      patients with higher circulating BMSC counts may be more resistant to septic and 
      traumatic insults. There are clear sex differences in response to such insults. 
      Within the population of BMSC, mesenchymal stem cells (MSCs) may have clinical 
      advantages. Therefore, we hypothesize that sex differences in the MSC paracrine 
      response to acute injury exist. Mesenchymal stem cells were obtained from male 
      and female mice. One million MSCs per well (triplicate wells per group) were 
      stressed by hypoxia and increasing doses of endotoxin (lipopolysaccharide [LPS]) 
      and hydrogen peroxide. Mesenchymal stem cell activation was determined by 
      measuring vascular endothelial growth factor (VEGF) and tumor necrosis factor 
      alpha production by enzyme-linked immunosorbent assay. Differences were 
      considered significant if P < 0.05. RESULTS: Lipopolysaccharide resulted in 
      significant activation of both male and female MSCs. However, LPS provoked 
      significantly more VEGF production in female MSCs versus male MSCs at all LPS 
      doses. Hypoxia of 1 h and hydrogen pyroxide exposure also caused significantly 
      more VEGF production in female MSCs versus male MSCs. Female MSCs expressed 
      significantly less tumor necrosis factor alpha than male MSCs after acute LPS and 
      hypoxia. CONCLUSION: This study constitutes the first demonstration that sex 
      differences exist in activated MSC function. Sex differences in progenitor cell 
      function may have important implications in understanding the observed sex 
      differences in the host's response to injury.
FAU - Crisostomo, Paul R
AU  - Crisostomo PR
AD  - Department of Surgery, Center for Immunobiology, Indiana University School of 
      Medicine, 545 Barnhill Drive, Indianapolis, IN 46202, USA.
FAU - Wang, Meijing
AU  - Wang M
FAU - Herring, Christine M
AU  - Herring CM
FAU - Morrell, Eric D
AU  - Morrell ED
FAU - Seshadri, Preethi
AU  - Seshadri P
FAU - Meldrum, Kirstan K
AU  - Meldrum KK
FAU - Meldrum, Daniel R
AU  - Meldrum DR
LA  - eng
GR  - R01 GM 070628/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Female
MH  - Hydrogen Peroxide/pharmacology
MH  - Lipopolysaccharides/metabolism/pharmacology
MH  - Male
MH  - Mesoderm/*cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Sex Factors
MH  - Stem Cells/*cytology
MH  - Stromal Cells/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2006/11/23 09:00
MHDA- 2007/01/18 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/01/18 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - 00024382-200612000-00007 [pii]
AID - 10.1097/01.shk.0000233195.63859.ef [doi]
PST - ppublish
SO  - Shock. 2006 Dec;26(6):571-4. doi: 10.1097/01.shk.0000233195.63859.ef.