PMID- 17118454
OWN - NLM
STAT- MEDLINE
DCOM- 20070329
LR  - 20061204
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 44
IP  - 8
DP  - 2007 Mar
TI  - Molecular cloning and characterization of Toll-like receptor 9 in Japanese 
      flounder, Paralichthys olivaceus.
PG  - 1845-53
AB  - Toll-like receptor (TLR) 9 cDNA and gene were cloned from Japanese flounder, 
      Paralichthys olivaceus. The Japanese flounder TLR9 cDNA encodes 1065 amino acids. 
      The leucine-rich domain (LRD) and the Toll/interleukin-1 receptor (TIR) domain 
      found in other vertebrate TLR9s were conserved in Japanese flounder TLR9. The 
      gene is composed of three exons and two introns. The Japanese flounder tumor 
      necrosis factor (TNF) gene promoter was activated in Japanese flounder 
      TLR9-transformed hirame natural embryo (HINAE) cells upon stimulation with 
      synthesized CpG oligodeoxynucleotide (ODN), but not by stimulation with GpC ODN. 
      The Japanese flounder TLR9 gene was highly expressed in epithelial and lymphoid 
      organs, such as the gills, intestines, kidney, spleen and stomach in an 
      apparently healthy fish. The mRNA copy numbers of Japanese flounder TLR9 and its 
      adapter protein, the myeloid differentiation factor 88 (MYD88) were increased in 
      some organs including blood, gill, kidney and spleen after Edwardsiella tarda 
      challenge. Immunohistochemical analysis revealed that TLR9 and MYD88 were 
      expressed in the same cells of kidney. Few TLR9-expressing cells were found in 
      gill, kidney and spleen in healthy Japanese flounder, but many were found in 
      these organs after E. tarda challenge and were coincident with lesions that had 
      been colonized by the bacteria.
FAU - Takano, Tomokazu
AU  - Takano T
AD  - Laboratory of Genome Science, Graduate School of Marine Science and Technology, 
      Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 
      108-8477, Japan.
FAU - Kondo, Hidehiro
AU  - Kondo H
FAU - Hirono, Ikuo
AU  - Hirono I
FAU - Endo, Makoto
AU  - Endo M
FAU - Saito-Taki, Tatsuo
AU  - Saito-Taki T
FAU - Aoki, Takashi
AU  - Aoki T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061121
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (DNA, Complementary)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Toll-Like Receptor 9)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Edwardsiella tarda/immunology
MH  - Enterobacteriaceae Infections/genetics/immunology/veterinary
MH  - Fish Diseases/genetics/immunology
MH  - Flounder/embryology/*genetics/immunology
MH  - Gene Expression Regulation/*physiology
MH  - Molecular Sequence Data
MH  - Myeloid Differentiation Factor 88/genetics/immunology
MH  - Organ Specificity/physiology
MH  - Protein Structure, Tertiary/genetics
MH  - Toll-Like Receptor 9/*genetics/immunology
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
EDAT- 2006/11/23 09:00
MHDA- 2007/03/30 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/08/04 00:00 [received]
PHST- 2006/10/05 00:00 [revised]
PHST- 2006/10/10 00:00 [accepted]
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/03/30 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - S0161-5890(06)00645-6 [pii]
AID - 10.1016/j.molimm.2006.10.018 [doi]
PST - ppublish
SO  - Mol Immunol. 2007 Mar;44(8):1845-53. doi: 10.1016/j.molimm.2006.10.018. Epub 2006 
      Nov 21.