PMID- 1711850
OWN - NLM
STAT- MEDLINE
DCOM- 19910731
LR  - 20061115
IS  - 0158-5231 (Print)
IS  - 0158-5231 (Linking)
VI  - 22
IP  - 5
DP  - 1990 Dec
TI  - Some yeast proteins are recognized by antibodies to reverse transcriptases from 
      several mammalian retroviruses and display reverse transcriptase activity.
PG  - 859-66
AB  - Antibodies directed against reverse transcriptases (RT) of the mammalian 
      retroviruses SSV and RD114 recognize specifically on Western Blots yeast 
      cytoplasmic soluble proteins of 31 and 45 kDa respectively and inhibit RT 
      activity in this fraction. Anti RLV RTIgG recognizes a protein of 40-43 kDa in 
      the particulate (VLP) fraction and inhibits RT activity in it. No inhibition of 
      RT activity was seen with normal serum anti AMV RTIgG or antisera against yeast 
      DNA polymerase I and DNA polymerase III. These yeast RTs display RNase H activity 
      and are not inhibited by aphidicolin. They prefer Mn+2 as cofactor over Mg+2, 
      display an optimum temperature of 25-30 degrees C and are expressed in a diploid 
      as well as 2 haploid strains and are thus distinct from yeast Ty encoded RTs.
FAU - Halbreich, A
AU  - Halbreich A
AD  - Laboratoire de Physiologie Cellulaire, Universite Pierre et Marie Curie, Paris, 
      France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Biochem Int
JT  - Biochemistry international
JID - 8100311
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Fungal Proteins)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Antibody Specificity
MH  - DNA Transposable Elements
MH  - Fungal Proteins/genetics/*immunology
MH  - Immunochemistry
MH  - RNA-Directed DNA Polymerase/*immunology
MH  - Retroviridae/*enzymology/immunology
MH  - Saccharomyces cerevisiae/*enzymology/genetics/immunology
EDAT- 1990/12/01 00:00
MHDA- 1990/12/01 00:01
CRDT- 1990/12/01 00:00
PHST- 1990/12/01 00:00 [pubmed]
PHST- 1990/12/01 00:01 [medline]
PHST- 1990/12/01 00:00 [entrez]
PST - ppublish
SO  - Biochem Int. 1990 Dec;22(5):859-66.