PMID- 17118604
OWN - NLM
STAT- MEDLINE
DCOM- 20070425
LR  - 20131121
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 43
IP  - 4
DP  - 2007 Mar 12
TI  - A rapid, simple, specific liquid chromatographic-electrospray mass spectrometry 
      method for the determination of finasteride in human plasma and its application 
      to pharmacokinetic study.
PG  - 1507-13
AB  - A fast, accurate, sensitive, selective and reliable method using reversed-phase 
      high-performance liquid chromatography-mass spectrometry coupling with an 
      electrospray ionization interface was developed and validated for the 
      determination of finasteride in human plasma. After deprotienation with 
      acetonitrile, centrifugation, evaporation to dryness and dissolving in mobile 
      phase, satisfactory separation was achieved on a Hypersil-Keystone C(18) 
      reversed-phase column using a mobile phase consisting of acetonitrile-water 
      (46:54, v/v), 0.1% acetic acid and 0.1% trifluoracetic acid. Carbamazepine (IS) 
      was used as internal standard. This method involved the use of the [M+H](+) ions 
      of finasteride and IS at m/z 373 and 237 with the selective ion monitoring (SIM) 
      mode. The calibration curve was linear in the range of 0.2-120 ng ml(-1). The 
      limit of quantification for finasteride in plasma was 0.2 ng ml(-1) with good 
      accuracy and precision. The intra-assay precision and accuracy were in the range 
      of 2.1-11.2% and -1.3% to 8.5%, respectively. The inter-assay precision and 
      accuracy were in the order of 3.4-12.1% and -1.5% to 11.5%, respectively. The 
      mean sample extract recoveries of the method were higher than 85% and 74% for 
      finasteride and internal standard (IS), respectively. The assay has been 
      successfully used to estimate the pharmacokinetics of finasteride after oral 
      administration of a 5mg tablet of finasteride to 24 healthy volunteers.
FAU - Guo, Fang-Qiu
AU  - Guo FQ
AD  - College of Chemistry and Chemical Engineering, Central South University, Changsha 
      410083, PR China.
FAU - Huang, Lan-Fang
AU  - Huang LF
FAU - Wong, Kin Ping
AU  - Wong KP
FAU - Dai, Yun-Hui
AU  - Dai YH
FAU - Li, Ya-Wen
AU  - Li YW
FAU - Liang, Yi-Zeng
AU  - Liang YZ
FAU - Huang, Ke-Long
AU  - Huang KL
FAU - Zhong, Ke-Jun
AU  - Zhong KJ
FAU - Wu, Ming-Jian
AU  - Wu MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061121
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Tablets)
RN  - 33CM23913M (Carbamazepine)
RN  - 57GNO57U7G (Finasteride)
SB  - IM
MH  - Administration, Oral
MH  - Adolescent
MH  - Adult
MH  - Carbamazepine/chemistry
MH  - Chromatography, Liquid/instrumentation/*methods
MH  - Enzyme Inhibitors/administration & dosage/*blood/chemistry/*pharmacokinetics
MH  - Finasteride/administration & dosage/*blood/chemistry/*pharmacokinetics
MH  - Humans
MH  - Male
MH  - Molecular Structure
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tablets
MH  - Time Factors
EDAT- 2006/11/23 09:00
MHDA- 2007/04/26 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/08/04 00:00 [received]
PHST- 2006/10/03 00:00 [revised]
PHST- 2006/10/17 00:00 [accepted]
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/04/26 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - S0731-7085(06)00717-5 [pii]
AID - 10.1016/j.jpba.2006.10.024 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2007 Mar 12;43(4):1507-13. doi: 10.1016/j.jpba.2006.10.024. 
      Epub 2006 Nov 21.