PMID- 17119118
OWN - NLM
STAT- MEDLINE
DCOM- 20070531
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 7
DP  - 2007 Apr 1
TI  - Arginase 1 is expressed in myelocytes/metamyelocytes and localized in gelatinase 
      granules of human neutrophils.
PG  - 3084-7
AB  - Arginase 1 (ARG1) metabolizes arginine, thus reducing the availability of 
      arginine as a substrate for nitric oxide synthase (NOS). The decreased production 
      of nitric oxide (NO) by NOS and the production of ornithine by ARG1 affect immune 
      responses and tissue regeneration at sites of infection, respectively. We here 
      demonstrate that ARG1 is synthesized in myelocytes/metamyelocytes and is stored 
      in gelatinase granules. In accordance with this, activated neutrophils coreleased 
      ARG1 and gelatinase to the extracellular environment on stimulation with 
      phorbol-12-myristate 13-acetate (PMA), formyl-methionyl-leucyl-phenylalanine 
      (fMLP), or tumor necrosis factor alpha (TNF-alpha). Overall, these findings 
      define ARG1 as a genuine gelatinase granule protein and support a model in which 
      activated neutrophils release ARG1 at sites of infection to modulate immune 
      responses and promote tissue regeneration.
FAU - Jacobsen, Lars C
AU  - Jacobsen LC
AD  - Granulocyte Research Laboratory, Department of Hematology, Rigshospitalet 
      University Hospital, Copenhagen, Denmark.
FAU - Theilgaard-Monch, Kim
AU  - Theilgaard-Monch K
FAU - Christensen, Erik I
AU  - Christensen EI
FAU - Borregaard, Niels
AU  - Borregaard N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.5.3.1 (Arginase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Arginase/*genetics/*metabolism
MH  - Cytoplasmic Granules/enzymology
MH  - Exocytosis
MH  - Gelatinases/metabolism
MH  - Granulocyte Precursor Cells/*enzymology
MH  - Humans
MH  - In Vitro Techniques
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*enzymology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2006/11/23 09:00
MHDA- 2007/06/01 09:00
CRDT- 2006/11/23 09:00
PHST- 2006/11/23 09:00 [pubmed]
PHST- 2007/06/01 09:00 [medline]
PHST- 2006/11/23 09:00 [entrez]
AID - S0006-4971(20)41817-8 [pii]
AID - 10.1182/blood-2006-06-032599 [doi]
PST - ppublish
SO  - Blood. 2007 Apr 1;109(7):3084-7. doi: 10.1182/blood-2006-06-032599.