PMID- 17120579 OWN - NLM STAT- MEDLINE DCOM- 20070116 LR - 20190715 IS - 0013-936X (Print) IS - 0013-936X (Linking) VI - 40 IP - 20 DP - 2006 Oct 15 TI - Enrichment, performance, and microbial diversity of a thermophilic mediatorless microbial fuel cell. PG - 6449-54 AB - A thermophilic mediatorless microbial fuel cell (ML-MFC) was developed for continuous electricity production while treating artificial wastewater concurrently. A maximum power density of 1030 +/- 340 mW/m2 was generated continuously at 55 degrees C with an anode retention time of 27 min (11 mL h(-1)) and continuous pumping of air-saturated phosphate buffer into the cathode compartment at the retention time of 0.7 min (450 mL h(-1)). Meanwhile, about 80% of the electrons available from acetate oxidation were recovered as current. Denaturing gradient gel electrophoresis (DGGE) and direct 16S-rRNA gene analysis revealed that the bacterial diversity in this ML-MFC system was lower than the inoculum. Direct 16S rDNA analysis showed that the dominant bacteria representing 57.8% of total population in anode compartment was phylogenetically very closely related to an uncultured clone, clone E4. Two sheets of graphite used as the anode showed different dominant bacterial population. For the first time, it is shown that thermophilic electrochemically active bacteria can be enriched to concurrently generate electricity and treat artificial wastewater in a thermophilic ML-MFC. FAU - Jong, Bor Chyan AU - Jong BC AD - Bioelectrochemistry Laboratory, Water Environment & Remediation Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea. FAU - Kim, Byung Hong AU - Kim BH FAU - Chang, In Seop AU - Chang IS FAU - Liew, Pauline Woan Ying AU - Liew PW FAU - Choo, Yeng Fung AU - Choo YF FAU - Kang, Gi Su AU - Kang GS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Environ Sci Technol JT - Environmental science & technology JID - 0213155 RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Sewage) SB - IM MH - Bacteria/classification/genetics/*growth & development MH - Biodiversity MH - Bioelectric Energy Sources/*microbiology MH - Electricity MH - Electrodes MH - Hot Temperature MH - RNA, Ribosomal, 16S/genetics MH - Sewage/microbiology EDAT- 2006/11/24 09:00 MHDA- 2007/01/17 09:00 CRDT- 2006/11/24 09:00 PHST- 2006/11/24 09:00 [pubmed] PHST- 2007/01/17 09:00 [medline] PHST- 2006/11/24 09:00 [entrez] AID - 10.1021/es0613512 [doi] PST - ppublish SO - Environ Sci Technol. 2006 Oct 15;40(20):6449-54. doi: 10.1021/es0613512.