PMID- 17120584
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20190715
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 40
IP  - 20
DP  - 2006 Oct 15
TI  - Development of the GENIPOL European flounder (Platichthys flesus) microarray and 
      determination of temporal transcriptional responses to cadmium at low dose.
PG  - 6479-88
AB  - We have constructed a high density, 13 270-clone cDNA array for the sentinel fish 
      species European flounder (Platichthys flesus), combining clones from suppressive 
      subtractive hybridization and a liver cDNA library; DNA sequences of 5211 clones 
      were determined. Fish were treated by single intraperitoneal injection with 50 
      micrograms cadmium chloride per kilogram body weight, a dose relevant to 
      environmental exposures, and hepatic gene expression changes were determined at 
      1, 2, 4, 8, and 16 days postinjection in comparison to saline-treated controls. 
      Gene expression responses were confirmed by real-time reverse transcription 
      polymerase chain reaction (RT-PCR). Blast2GO gene ontology analysis highlighted a 
      general induction of the unfolded protein response, response to oxidative stress, 
      protein synthesis, transport, and degradation pathways, while apoptosis, cell 
      cycle, cytoskeleton, and cytokine genes were also affected. Transcript levels of 
      cytochrome P450 1A (CYP1A) were repressed and vitellogenin altered, real-time PCR 
      showed induction of metallothionein. We thus describe the establishment of a 
      useful resource for ecotoxicogenomics and the determination of the temporal 
      molecular responses to cadmium, a prototypical heavy metal pollutant.
FAU - Williams, Tim D
AU  - Williams TD
AD  - School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 
      2TT UK. t.d.williams@bham.ac.uk
FAU - Diab, Amer M
AU  - Diab AM
FAU - George, Stephen G
AU  - George SG
FAU - Godfrey, Rita E
AU  - Godfrey RE
FAU - Sabine, Victoria
AU  - Sabine V
FAU - Conesa, Ana
AU  - Conesa A
FAU - Minchin, Steven D
AU  - Minchin SD
FAU - Watts, Phil C
AU  - Watts PC
FAU - Chipman, James K
AU  - Chipman JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Animals
MH  - Cadmium Chloride/administration & dosage/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Environmental Monitoring/methods
MH  - Flounder/*genetics/growth & development
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Liver/drug effects/growth & development/metabolism
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic/*drug effects
EDAT- 2006/11/24 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/11/24 09:00
PHST- 2006/11/24 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/11/24 09:00 [entrez]
AID - 10.1021/es061142h [doi]
PST - ppublish
SO  - Environ Sci Technol. 2006 Oct 15;40(20):6479-88. doi: 10.1021/es061142h.