PMID- 17120748
OWN - NLM
STAT- MEDLINE
DCOM- 20080606
LR  - 20211103
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 26
IP  - 4
DP  - 2006
TI  - Effect of icariin on cyclic GMP levels and on the mRNA expression of cGMP-binding 
      cGMP-specific phosphodiesterase (PDE5) in penile cavernosum.
PG  - 460-2
AB  - To further investigate the mechanisms of action of icariin (ICA), we assessed the 
      effects of ICA on the in vitro formation of cGMP and cAMP in isolated rabbit 
      corpus cavernosum. Isolated segments of rabbit corpus cavernosum were exposed to 
      increasing concentrations of ICA and the dose-dependent accumulation of cGMP and 
      cAMP was determined in the tissues samples by means of 125I radioimmunoassay. 
      Responses of the isolated tissues preparations to ICA were compared with those 
      obtained with the reference compounds sildenafil (Sild). Furthermore, the effects 
      of ICA on the mRNA expression of specific cGMP-binding phosphodiesterase type V 
      (PDE5) in rat penis were also observed. After incubation with ICA for 6 h or 14 h 
      respectively, the levels of PDE5 mRNA were examined by reverse transcriptase 
      polymerase chain reaction (RT-PCR). The results showed that ICA increased cGMP 
      concentrations directly (P < 0.05), but there was no significant effect on cAMP 
      concentrations (P > 0.05). In the presence of sodium nitroprusside (SNP), a 
      stimulatory agent of cGMP, both ICA and Sild increased cGMP concentrations with 
      increasing dose (P < 0.01). Their EC50 was 4.62 (ICA) and 0.42 (Sild) micromol/L 
      respectively. Under the same condition, ICA and Sild unaltered cAMP level 
      significantly (P > 0.05). There were PDE5A1 and PDE5A2 mRNA expressions in rat 
      corpus cavernosum with PDE5A2 being the dominant isoform. ICA could obviously 
      inhibit these two isoforms mRNA expression in rat penis, and decrease PDE5A1 more 
      pronouncedly (P < 0.01). The present study indicated that the aphrodisiac 
      mechanisms of icariin involved the NO-cGMP signal transduction pathway, with 
      increasing cGMP levels in the corpus cavernosum smooth muscle. The inhibitory 
      effect of icariin on PDE5 mRNA expression, especially on PDE5A1, might account 
      for its molecular mechanisms for its long-term activity.
FAU - Jiang, Zhaojian
AU  - Jiang Z
AD  - Department of Pharmacology, School of Basic Medical Sciences, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan 430030, China.
FAU - Hu, Benrong
AU  - Hu B
FAU - Wang, Jialing
AU  - Wang J
FAU - Tang, Qiang
AU  - Tang Q
FAU - Tan, Yan
AU  - Tan Y
FAU - Xiang, Jizhou
AU  - Xiang J
FAU - Liu, Juyan
AU  - Liu J
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (RNA, Messenger)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.1.4.35 (Cyclic Nucleotide Phosphodiesterases, Type 5)
RN  - EC 3.1.4.35 (Pde5a protein, rat)
RN  - H2D2X058MU (Cyclic GMP)
RN  - VNM47R2QSQ (icariin)
SB  - IM
MH  - Animals
MH  - Cyclic AMP/metabolism
MH  - Cyclic GMP/*metabolism
MH  - Cyclic Nucleotide Phosphodiesterases, Type 5/*biosynthesis/genetics
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/pharmacology
MH  - Flavonoids/*pharmacology
MH  - In Vitro Techniques
MH  - Male
MH  - Penis/*drug effects/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rabbits
MH  - Radioimmunoassay
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2006/11/24 09:00
MHDA- 2008/06/07 09:00
CRDT- 2006/11/24 09:00
PHST- 2006/11/24 09:00 [pubmed]
PHST- 2008/06/07 09:00 [medline]
PHST- 2006/11/24 09:00 [entrez]
AID - 10.1007/s11596-006-0421-y [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2006;26(4):460-2. doi: 
      10.1007/s11596-006-0421-y.